Curative effects of Distemonanthus benthamianus Baillon. Trunk-bark extracts on enteropathogenic Escherichia coli 31-induced diarrhea in rats
| Autor: | Yousseu Nana William, Kamanyi Albert, Taous Khan, Fazli Wahid, Ateufack Gilbert, Abdul Jabbar Shah, Kashif Bashir, Jules-Roger Kuiate, Muhammad Yameen, Wasim Sajjad, Syed Luqman Shah, Mbiantcha Marius |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Diarrhea Klebsiella pneumoniae Ileum Microbial Sensitivity Tests 010502 geochemistry & geophysics 01 natural sciences High-performance liquid chromatography Enterococcus faecalis Microbiology 03 medical and health sciences chemistry.chemical_compound Enteropathogenic Escherichia coli medicine Animals Gallic acid Rats Wistar Chromatography High Pressure Liquid Escherichia coli Infections 0105 earth and related environmental sciences biology Chemistry Plant Extracts Fabaceae biology.organism_classification Proteus mirabilis Anti-Bacterial Agents Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Complementary and alternative medicine Plant Bark medicine.symptom |
| Zdroj: | Journal of complementaryintegrative medicine. 16(4) |
| ISSN: | 1553-3840 |
| Popis: | Background Distemonanthus benthamianus is used in the Western part of Cameroon to treat diarrheal episodes and infections. This study assessed its trunk-bark extracts activity against enteropathogenic Escherichia coli 31 (EPEC 31)-induced diarrhea in rats. Methods Aqueous and methanolic extracts were analyzed through high-performance liquid chromatography (HPLC). In vitro minimum inhibitory and bactericidal concentrations (MICs/MBCs) were evaluated on Enterococcus faecalis (ATCC 10,541), E. coli (ATCC 6539), Klebsiella pneumoniae (ATCC 13,883), Salmonella typhi (ATCC 6539) strains and on Proteus mirabilis, Pseudomonas aeruginosa (PA 01) and Shigella flexneri isolates using the microdilution method. Diarrhea was induced by inoculating rats with EPEC 31 (1.5 × 108 CFU/mL; p.o). Serum transaminases level assay and enzyme-linked immunosorbent assay (ELISA) for cytokines determination were performed. Hematoxylin–eosin (H–E) staining was used for intestinal tissue analysis. Results HPLC fingerprints of extracts showed presence of gallic acid and other unidentified compounds. The lowest MIC of 256 µg/mL was obtained with methanolic extract. At 100 mg/kg, both extracts significantly (p Conclusion These findings suggest that D. benthamianus trunk-bark extracts displayed therapeutic effects against infectious diarrhea in rats. |
| Databáze: | OpenAIRE |
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