Development and validation of a potentiometric biosensor assay for tylosin with demonstrated applicability for the detection of two other antimicrobial growth-promoter compounds in feedstuffs

Autor:	Phil Teale, K.B. Wolodko-Cierniak, O. Leonardova, D. Purvis, S.L. Stead, Matthew Sharman, S.F. Richmond, P. Driver
Rok vydání:	2011
Předmět:	Food Safety Potentiometric biosensor Animal feed Health Toxicology and Mutagenesis Coefficient of variation Food Contamination Biosensing Techniques Tylosin Toxicology Mass spectrometry Virginiamycin chemistry.chemical_compound Spiramycin Animals Humans Bioassay Measurement repeatability Growth Substances Chromatography Chemistry Public Health Environmental and Occupational Health Veterinary Drugs General Chemistry General Medicine Antimicrobial Animal Feed Anti-Bacterial Agents Potentiometry Food Science
Zdroj:	Food Additives & Contaminants: Part A. 28:848-859
ISSN:	1944-0057 1944-0049
DOI:	10.1080/19440049.2011.567399
Popis:	A potentiometric biosensor assay based on a commercially available polyclonal antibody was developed to detect tylosin residues in animal feed. The method can be used as a rapid (less than 45 min) laboratory-based procedure or as a portable field-test for the simultaneous measurement of up to 12 different samples. For both procedures the qualitative detection capability (CCβ) for tylosin was determined as 0.2 mg kg(-1) in a range of animal feeds with a measurement repeatability at concentrations between 0.2 and 4 mg kg(-1) of ≤13% coefficient of variation (%CV). The field-test format was capable of detecting tylosin residues at operating (external air) temperatures ranging between +4 and 37°C, although some reduction in signal was observed at the lower temperatures. The laboratory-based tylosin assay was evaluated using 16 medicated and 22 non-medicated feeds and was found to give comparable data with a confirmatory method based upon liquid chromatography-tandem mass spectrometry (LC-MS/MS). The potential to develop a multi-probe format assay for the simultaneous detection of tylosin, spiramycin and virginiamycin was also demonstrated. Cross-validation in a second laboratory showed the assay to be transferable, reliable and robust.
Databáze:	OpenAIRE
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