Single nucleotide polymorphism of CD40 in the 5'-untranslated region is associated with ischemic stroke
| Autor: | Shun-Xian Wang, Bo Zhang, Guo-Guang Peng, Xiaoming Wang, Bi-Hua Wu, Ju-Ming Yu, Ying Ma, Yun Liu |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Linkage disequilibrium medicine.medical_specialty Single-nucleotide polymorphism Biology Gastroenterology Polymorphism Single Nucleotide Brain Ischemia Brain ischemia Internal medicine Genotype Genetics Genetic predisposition medicine SNP Humans CD40 Antigens Genetic Association Studies Aged Case-control study General Medicine Odds ratio Middle Aged medicine.disease Stroke Case-Control Studies Female 5' Untranslated Regions |
| Zdroj: | Gene. 529(2) |
| ISSN: | 1879-0038 |
| Popis: | Objectives Ischemic stroke is influenced by both environmental and genetic factors. The CD40/CD40L system is related to proinflammatory and prothrombogenic responses, which are involved in the pathophysiology of ischemic stroke. The aim of this study was to evaluate association between the CD40 -1C/T single nucleotide polymorphism (SNP) and ischemic stroke in a Chinese population. Methods We conducted a case–control study including 286 ischemic stroke patients and 336 controls. CD40 -1C/T SNP was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing methods, and evaluated its relevance to ischemic stroke susceptibility. Results Significantly increased ischemic stroke risk was found to be associated with the T allele of CD40 -1C/T (OR = 1.273, 95% CI = 1.016–1.594). The frequencies of CT and TT/CT genotypes of CD40 -1C/T in ischemic stroke patients were significantly higher than those of controls, respectively (for CT: OR = 2.350, 95% CI = 1.601–3.449; for TT/CT: OR = 2.148, 95% CI = 1.479–3.119). And, similar results were obtained after adjusting non-matched variables. We found that the frequency of carried T genotypes (TT and TT/CT) was significantly increased in patients with history of stroke compared with patients without (for TT: OR = 6.538, 95%CI = 1.655–25.833; for TT/CT: OR = 3.469, 95%CI = 1.031–11.670), respectively. Conclusions The findings suggested that the CD40 -1C/T polymorphism might contribute to the susceptibility to ischemic stroke in the Chinese population, and might be associated with history of previous stroke. |
| Databáze: | OpenAIRE |
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