Gamete quality and management for in vitro fertilisation in meagre (Argyrosomus regius)

Autor: Constantinos C. Mylonas, Christian Fauvel, Wendy González, Neil Duncan, Sandra Ramos-Júdez, Gilbert Dutto
Přispěvatelé: MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Producció Animal, Aqüicultura
Rok vydání: 2019
Předmět:
water volume
Artificial fertilization
hatching rates
media_common.quotation_subject
medicine.medical_treatment
Aquatic Science
Argyrosomus regius
artificial fertilization
cultured fish
Andrology
03 medical and health sciences
GnRHa
[SDV.EE.ECO]Life Sciences [q-bio]/Ecology
environment/Ecosystems
medicine
14. Life underwater
injections
Ovulation
time
Fertilisation
030304 developmental biology
media_common
Artificial fertilisation
0303 health sciences
In vitro fertilisation
Meagre
biology
Reproduction
sperm activation
Gamete management
04 agricultural and veterinary sciences
biology.organism_classification
Oocyte
Sperm
medicine.anatomical_structure
ovulation
040102 fisheries
0401 agriculture
forestry
and fisheries
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
spermatozoaoocyte ratio
sea-bass
Spermatogenesis
Zdroj: Aquaculture
Aquaculture, Elsevier, 2019, 509, pp.227--235. ⟨10.1016/j.aquaculture.2019.05.033⟩
Aquaculture (0044-8486) (Elsevier BV), 2019-07, Vol. 509, P. 227-235
IRTA Pubpro. Open Digital Archive
Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
ISSN: 0044-8486
Popis: The aquaculture of meagre (Argyrosomus regius) requires methods for the control of reproduction that enable the production of families from specific individuals for selective breeding programs. We experimentally determined the parameters required for an in vitro fertilisation protocol. A total of 14 females and 5 males (mean ± S.D. weights of 20.45 ± 6.22 and 15.94 ± 2.75 kg, respectively) were used. Selected females had vitellogenic oocytes >550 μm in diameter and males had fluid sperm upon application of abdominal pressure. Both sexes were treated with an injection of 15 μg kg−1 of gonadotropin-releasing hormone agonist (GnRHa) to induce oocyte maturation/ovulation and enhance sperm production. To determine the timing of ovulation and window of high egg viability, females were stripped serially every 2.5 h beginning 35 h after GnRHa treatment. Sperm was obtained 24 h after GnRHa treatment and was diluted 1/4 in modified Leibovitz for storage at 4 °C until use. Sperm quality parameters such as percentage initial spermatozoa motility, duration of motility, velocity and density were determined using computer assisted sperm analysis (CASA). In vitro inseminations were made in duplicate or triplicate batches of eggs from each spawn by mixing 0.5–1 mL of eggs, 20–40 μL diluted sperm (pooled from two males) and 100 mL of seawater. Fertilisation success was examined at spermatozoa (spz): egg ratios between ~2000 and 400,000 spz egg−1. The optimal time for stripping ovulated females was ≤3 h after ovulation, which was the window of optimal egg viability. Ovulation under the conditions of this study was close to 38 h after GnRHa treatment, with a range from 35 to 41 h. Beginning from 3 h after ovulation, egg viability declined probably due to overripening. Sperm diluted in Leibovitz maintained motility and velocity for as long as 7 h after collection. Spermatozoa motility (%) and average path velocity (VAP, μm/s) of sperm samples obtained from males before GnRHa injection declined rapidly after activation compared to the samples obtained 24 h post-injection, with significant decreases respectively after 75 and 45 s. A minimum ratio of 150,000 spermatozoa egg−1 was necessary to ensure high fertilisation success. The acquired knowledge of the present study will aid the aquaculture industry and future research on selective breeding programs for meagre. info:eu-repo/semantics/acceptedVersion
Databáze: OpenAIRE
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