Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women
| Autor: | Jan Polak, Isabelle de Glisezinski, Marie Adeline Marques, Katrien Koppo, Dominique Langin, Michel Berlan, Michaela Siklova-Vitkova, Vladimir Stich, Jens Bülow, Johan Van de Voorde, Eva Klimcakova |
|---|---|
| Přispěvatelé: | Department of Sports Medicine, Charles University [Prague] (CU), Department of Pharmacology, Universiteit Gent = Ghent University [Belgium] (UGENT), Franco-czech Laboratory for clinical research on obesity, Charles University [Prague] (CU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Clinical Physiology, Bispebjerg Hospital-Universiy of Copenhagen, Service de biochimie clinique, CHU Toulouse [Toulouse], Service de médecine du sport, Simon, Marie Francoise, Universiteit Gent = Ghent University (UGENT), Laboratoire de Biochimie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Médecine du Sport [CHU Toulouse], Département Chirurgie Orthopédique et traumatologique [CHU Toulouse], Pôle Inflammation, infection, immunologie et loco-moteur [CHU Toulouse] (Pôle I3LM Toulouse), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Inflammation, infection, immunologie et loco-moteur [CHU Toulouse] (Pôle I3LM Toulouse), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Physiology
Endocrinology Diabetes and Metabolism medicine.medical_treatment Adipose tissue Adrenergic MESH: Glucose Clamp Technique 0302 clinical medicine Catecholamines Weight loss Medicine Insulin MESH: Obesity [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism MESH: Diet Reducing 0303 health sciences Glucose clamp technique [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism MESH: Insulin Resistance Epinephrine Adipose Tissue Female medicine.symptom MESH: Adipose Tissue medicine.drug Adult medicine.medical_specialty Diet Reducing Lipolysis Calorie restriction 030209 endocrinology & metabolism MESH: Insulin 03 medical and health sciences MESH: Weight Loss Physiology (medical) Internal medicine Weight Loss MESH: Catecholamines Humans MESH: Lipolysis Obesity 030304 developmental biology Caloric Restriction MESH: Caloric Restriction MESH: Humans business.industry MESH: Adult Endocrinology Glucose Clamp Technique Insulin Resistance business MESH: Female |
| Zdroj: | AJP-Endocrinology and Metabolism AJP-Endocrinology and Metabolism, American Physiological Society, 2012, 302 (2), pp.E226-32. ⟨10.1152/ajpendo.00240.2011⟩ AJP-Endocrinology and Metabolism, 2012, 302 (2), pp.E226-32. ⟨10.1152/ajpendo.00240.2011⟩ |
| ISSN: | 0193-1849 1522-1555 |
| DOI: | 10.1152/ajpendo.00240.2011⟩ |
| Popis: | The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the α2-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|