Effect of 5-aza-2'-deoxycytidine on cell proliferation of non- small cell lung cancer cell line A549 cells and expression of the TFPI-2 gene
| Autor: | Shui-Bo Zhu, Yong-Qiang Dong, Gui-Lin Yin, Jiang-Shui Liang, Tao Ji, Jia-Hang Xu, Xiao-ming Zhang |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Antimetabolites Antineoplastic Lung Neoplasms Epidemiology Blotting Western Biology Real-Time Polymerase Chain Reaction Carcinoma Non-Small-Cell Lung Gene expression Tumor Cells Cultured Humans MTT assay RNA Messenger Cell Proliferation Glycoproteins A549 cell Cell growth Reverse Transcriptase Polymerase Chain Reaction Cell Cycle Public Health Environmental and Occupational Health Cell cycle Flow Cytometry Molecular biology Real-time polymerase chain reaction Oncology Cell culture DNA methylation Azacitidine |
| Zdroj: | Asian Pacific journal of cancer prevention : APJCP. 14(7) |
| ISSN: | 2476-762X |
| Popis: | Objective: The present study employed 5-aza-2'-deoxycytidine (5-Aza-CdR) to treat non-small cell lung cancer (NSCLC) cell line A549 to investigate the effects on proliferation and expression of the TFPI-2 gene. Methods: Proliferation was assessed by MTT assay after A549 cells were treated with 0, 1, 5, 10 μmol/L 5-Aza-CdR, a specific demethylating agent, for 24 ,48 and 72h. At the last time point cells were also analyzed by flow cytometry (FCM) to identify any change in their cell cycle profiles. Methylation-specific polymerase chain reaction (MSPCR), real time polymerase chain reaction(real-time PCR) and western blotting were carried out to determine TFPI-2 gene methylation status, mRNA expression and protein expression. Results: MTT assay showed that the growth of A549 cells which were treated with 5-Aza-CdR was significantly suppressed as compared with the control group (0 μmol/L 5-Aza-CdR). After treatment with 0, 1, 5, 10 μmol/L 5-Aza-CdR for 72h, FCM showed their proportion in G0/G1 was 69.7±0.99%, 76.1±0.83%, 83.8±0.35%, 95.5±0.55% respectively (P |
| Databáze: | OpenAIRE |
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