Efficient Cellular Release of Rift Valley Fever Virus Requires Genomic RNA
| Autor: | Sonja R. Gerrard, Mary E. Piper, Dorothy R. Sorenson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
viruses
lcsh:Medicine RNA-dependent RNA polymerase Fluorescent Antibody Technique Biology Virus Replication Genome Biochemistry Microbiology Virus Cell Line 03 medical and health sciences chemistry.chemical_compound VP40 Microscopy Electron Transmission RNA polymerase Animals lcsh:Science 030304 developmental biology DNA Primers Infectivity 0303 health sciences Multidisciplinary Base Sequence 030306 microbiology Reverse Transcriptase Polymerase Chain Reaction lcsh:R RNA RNA-Dependent RNA Polymerase Rift Valley fever virus Virology 3. Good health Infectious Diseases Viral replication chemistry Veterinary Diseases Biocatalysis Medicine lcsh:Q Veterinary Science Ultracentrifugation Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 3, p e18070 (2011) |
| ISSN: | 1932-6203 |
| Popis: | The Rift Valley fever virus is responsible for periodic, explosive epizootics throughout sub-Saharan Africa. The development of therapeutics targeting this virus is difficult due to a limited understanding of the viral replicative cycle. Utilizing a virus-like particle system, we have established roles for each of the viral structural components in assembly, release, and virus infectivity. The envelope glycoprotein, Gn, was discovered to be necessary and sufficient for packaging of the genome, nucleocapsid protein and the RNA-dependent RNA polymerase into virus particles. Additionally, packaging of the genome was found to be necessary for the efficient release of particles, revealing a novel mechanism for the efficient generation of infectious virus. Our results identify possible conserved targets for development of anti-phlebovirus therapies. |
| Databáze: | OpenAIRE |
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