Prevalence and characteristics of verotoxin-producing Escherichia coli (VTEC) in stool samples from asymptomatic human carriers working in the meat processing industry in Switzerland
| Autor: | R. Stephan, F. Untermann, S. Ragettli |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Serotype
Food Handling Bacterial Toxins Virulence Enterotoxin Biology Escherichia coli O157 Shiga Toxin 1 medicine.disease_cause Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Feces fluids and secretions Genotype Escherichia coli Prevalence medicine Humans Serotyping Meat-Packing Industry Escherichia coli Infections Intimin General Medicine Virology Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field VTEC Carrier State Asymptomatic carrier Switzerland Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 88:335-341 |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1046/j.1365-2672.2000.00965.x |
| Popis: | A total of 5590 stool samples from healthy employees in the meat industry were screened by PCR for verotoxin-producing Escherichia coli (VTEC). The PCR product of VT-encoding genes was detected in 3. 5% of the samples. Phenotypic and genotypic traits of 47 VTEC strains isolated from asymptomatic carriers were characterized. A variety of serotypes was found; one strain belonged to the serotype O157:H7. The majority of the isolates proved to be VT2-positive. Fifty-seven percent of the verotoxin-producing strains harboured the genes for one or several additional virulence associated factors, including intimin (eae, 8.5%), the 60 MDa plasmid (42.5%), enterohaemolysin (EHEC-hlyA, 38.3%), the heat-stable enterotoxin (astA, 6.4%), a serin protease (espP, 6.4%), colicin production (col D157, 12.8%) and a secretion system II (etpD, 10.6%). None of the strains was positive for a specific enzyme with catalase-peroxidase activity (katP). |
| Databáze: | OpenAIRE |
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