Ancient DNA from 8400 Year-Old Çatalhöyük Wheat: Implications for the Origin of Neolithic Agriculture
| Autor: | Hatice Bilgiç, Mahinur S. Akkaya, Anamika Pandey, Erdogan E. Hakki, Mohd Kamran Khan |
|---|---|
| Přispěvatelé: | Selçuk Üniversitesi |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Time Factors DNA Plant Turkey Science Plant genetics Polymerase Chain Reaction 03 medical and health sciences Glutenin Species Specificity Botany Domestication Promoter Regions Genetic History Ancient Phylogeny Triticum 2. Zero hunger Multidisciplinary biology business.industry food and beverages Agriculture Molecular Weight 030104 developmental biology Ancient DNA Archaeology Seeds biology.protein Medicine Autoradiography business |
| Zdroj: | PLoS ONE, Vol 11, Iss 3, p e0151974 (2016) |
| ISSN: | 1932-6203 |
| Popis: | WOS: 000372694700089 PubMed: 26998604 Human history was transformed with the advent of agriculture in the Fertile Crescent with wheat as one of the founding crops. Although the Fertile Crescent is renowned as the center of wheat domestication, archaeological studies have shown the crucial involvement of Catalhoyuk in this process. This site first gained attention during the 1961-65 excavations due to the recovery of primitive hexaploid wheat. However, despite the seeds being well preserved, a detailed archaeobotanical description of the samples is missing. In this article, we report on the DNA isolation, amplification and sequencing of ancient DNA of charred wheat grains from Catalhoyuk and other Turkish archaeological sites and the comparison of these wheat grains with contemporary wheat species including T. monococcum, T. dicoccum, T. dicoccoides, T. durum and T. aestivum at HMW glutenin protein loci. These ancient samples represent the oldest wheat sample sequenced to date and the first ancient wheat sample from the Middle East. Remarkably, the sequence analysis of the short DNA fragments preserved in seeds that are approximately 8400 years old showed that the Catalhoyuk wheat stock contained hexaploid wheat, which is similar to contemporary hexaploid wheat species including both naked (T. aestivum) and hulled (T. spelta) wheat. This suggests an early transitory state of hexaploid wheat agriculture from the Fertile Crescent towards Europe spanning present-day Turkey. TUBITAK (The Scientific and Technical Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG 22045, 101T046]; United Nations Educational, Scientific and Cultural Organization (UNESCO) The present research work was funded by TUBITAK (The Scientific and Technical Research Council of Turkey) under project No. TBAG 22045 and 101T046. The authors thank United Nations Educational, Scientific and Cultural Organization (UNESCO) for providing a scholarship to HB for conducting second laboratory aDNA isolation at UMIST Biomolecular Sciences, Manchester, UK. |
| Databáze: | OpenAIRE |
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