Autoantibody to scaffold attachment factor B (SAFB): A novel connective tissue disease-related autoantibody associated with interstitial lung disease
| Autor: | Masahide Yasui, Manabu Fujimoto, Yasuhito Hamaguchi, Kazuhiko Takehara, Mariko Seishima, Tomoya Asano, Akiko Takeuchi, Masataka Kuwana, Yoshinobu Okamoto, Takashi Matsushita, Masayoshi Hirata, Kenzo Kaji, Akira Higashi, Naoto Oishi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Immunology Enzyme-Linked Immunosorbent Assay Polymyositis Autoantigens 03 medical and health sciences 0302 clinical medicine Nuclear Matrix-Associated Proteins medicine Eosinophilic pneumonia Immunology and Allergy Humans Connective Tissue Diseases Aged Autoantibodies 030203 arthritis & rheumatology business.industry Interstitial lung disease Autoantibody Scaffold attachment factor B Overlap syndrome Matrix Attachment Region Binding Proteins Dermatomyositis Middle Aged medicine.disease Connective tissue disease 030104 developmental biology Phenotype Receptors Estrogen Case-Control Studies Female business Lung Diseases Interstitial Biomarkers |
| Zdroj: | Journal of autoimmunity. 76 |
| ISSN: | 1095-9157 |
| Popis: | Objective To identify and characterize a novel connective tissue disease (CTD)-related autoantibody (autoAb) directed against scaffold attachment factor B (SAFB). Methods AutoAb specificity was analyzed using RNA and protein-immunoprecipitation assays. Autoimmune targets were affinity purified using patients' sera and subjected to liquid chromatography mass spectrometry. Results By immunoprecipitation assay, 10 sera reacted with a protein with a molecular weight of approximately 160 kDa. Liquid chromatography mass spectrometry of the partially purified autoantigen and additional immunoblot-based analyses revealed that the Ab specifically recognized SAFB. Anti-SAFB Abs were detected in 2 of 646 patients with systemic sclerosis (SSc) (0.3%), 1 of 1570 patients with polymyositis/dermatomyositis (0.06%), 4 of 270 patients with interstitial lung disease (ILD) (1.5%), 1 of 43 patients with overlap syndrome (2.3%) and 2 patients with other diseases including primary Raynaud's disease and eosinophilic pneumonia. Five patients with anti-SAFB Abs had Raynaud's phenomenon and 3 had nail fold punctate hemorrhage. Of note, 8 of the 10 patients (80%) suffered from ILD. None of the patients with anti-SAFB Abs had pulmonary arterial hypertension, heart disease, or renal involvement. Conclusions Anti-SAFB Ab is a novel CTD-related autoAb possibly associated with ILD. |
| Databáze: | OpenAIRE |
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