RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes
| Autor: | Yuki Matsuba, Eran Pichersky, Vasiliki Falara, Michiel T.J. de Both, Paul Johan Diergaarde, Joerg Bohlmann, Michel A. Haring, Petra M. Bleeker, Eleni A. Spyropoulou, Robert C. Schuurink, Hanne Volpin, Philipp Zerbe |
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| Přispěvatelé: | Plant Physiology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
DNA Complementary Recombinant Fusion Proteins Plant Science Biology Solanum Sesquiterpene 01 natural sciences Article Terpene 03 medical and health sciences chemistry.chemical_compound Solanum lycopersicum Jasmonate Valencene Botany Genetics Wild tomato Gene Library Plant Proteins 030304 developmental biology 2. Zero hunger 0303 health sciences Alkyl and Aryl Transferases Sequence Analysis RNA Jasmonic acid fungi food and beverages Trichomes General Medicine biology.organism_classification Trichome chemistry Biochemistry RNA Plant Wild and cultivated tomato Sesquiterpene synthase RNA-seq Sesquiterpenes Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Molecular Biology, 77(4-5), 323-336. Springer Netherlands Plant Molecular Biology |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | Solanum lycopersicum and Solanum habrochaites (f. typicum) accession PI127826 emit a variety of sesquiterpenes. To identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing (RNA-seq) to obtain the transcriptome of the stem trichomes from these plants. This approach resulted initially in the discovery of six sesquiterpene synthase cDNAs from S. lycopersicum and five from S. habrochaites. Searches of other databases and the S. lycopersicum genome resulted in the discovery of two additional sesquiterpene synthases expressed in trichomes. The sesquiterpene synthases from S. lycopersicum and S. habrochaites have high levels of protein identity. Several of them appeared to encode for non-functional proteins. Functional recombinant proteins produced germacrenes, β-caryophyllene/α-humulene, viridiflorene and valencene from (E,E)-farnesyl diphosphate. However, the activities of these enzymes do not completely explain the differences in sesquiterpene production between the two tomato plants. RT-qPCR confirmed high levels of expression of most of the S. lycopersicum sesquiterpene synthases in stem trichomes. In addition, one sesquiterpene synthase was induced by jasmonic acid, while another appeared to be slightly repressed by the treatment. Our data provide a foundation to study the evolution of terpene synthases in cultivated and wild tomato. Electronic supplementary material The online version of this article (doi:10.1007/s11103-011-9813-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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