Troxerutin attenuates myocardial cell apoptosis following myocardial ischemia-reperfusion injury through inhibition of miR-146a-5p expression
| Autor: | Gongcheng Huang, Chen Huang, Liliang Shu, Zhu Xiaohua, Jing Xu, Gang Su, Wanzhe Zhang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Troxerutin Physiology Clinical Biochemistry Down-Regulation Apoptosis Myocardial Reperfusion Injury Pharmacology 03 medical and health sciences chemistry.chemical_compound Phosphatidylinositol 3-Kinases 0302 clinical medicine Western blot Downregulation and upregulation Lactate dehydrogenase medicine Animals Myocytes Cardiac Rats Wistar TUNEL assay medicine.diagnostic_test Chemistry Myocardium Hemodynamics Cell Biology medicine.disease Hydroxyethylrutoside MicroRNAs 030104 developmental biology Terminal deoxynucleotidyl transferase Animals Newborn Gene Expression Regulation 030220 oncology & carcinogenesis Reperfusion injury Proto-Oncogene Proteins c-akt medicine.drug |
| Zdroj: | Journal of cellular physiology. 234(6) |
| ISSN: | 1097-4652 |
| Popis: | The aim of the current study was to investigate the effects and the underlying mechanisms of troxerutin on myocardial cell apoptosis during ischemia-reperfusion (I/R) injury. Hypoxia/reoxygenation (H/R) model in neonatal rat cardiomyocytes, and I/R model in rats, were established following troxerutin preconditioning. The quantitative real-time polymerase chain reaction analysis was performed to examine the messenger RNA miR-146a-5p expression in cardiomyocytes and myocardial tissues. Hemodynamic parameters and serum creatine kinase, lactate dehydrogenase, tumor necrosis factor-α, and interleukin-10 were evaluated. Infarct size was examined by 2,3,5-triphenyltetrazolium chloride staining. Besides, myocardial apoptosis was detected by terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) assay. Western blot analysis was performed to determine the protein levels of caspase-3, Bax, and Bcl-2. The results showed that, troxerutin decreased rat cardiomyocyte apoptosis during H/R injury. Furthermore, the antiapoptotic effect of troxerutin against I/R injury was mediated by miR-146a-5p downregulation. In vivo experiments suggested that troxerutin alleviated myocardial I/R injury in rats via inhibition of miR-146a-5p. In conclusion, troxerutin exerted cardioprotective effects during I/R injury by downregulating miR-146a-5p. |
| Databáze: | OpenAIRE |
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