RNase III CLASH in MRSA uncovers sRNA regulatory networks coupling metabolism to toxin expression
| Autor: | Stuart W. McKellar, Ivayla Ivanova, Pedro Arede, Rachel L. Zapf, Noémie Mercier, Liang-Cui Chu, Daniel G. Mediati, Amy C. Pickering, Paul Briaud, Robert G. Foster, Grzegorz Kudla, J. Ross Fitzgerald, Isabelle Caldelari, Ronan K. Carroll, Jai J. Tree, Sander Granneman |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
RNA
Messenger/genetics Methicillin-Resistant Staphylococcus aureus Ribonuclease III Multidisciplinary RNA processing sRNA RNA-RNA interactions CLASH RNase E Staphylococcus aureus MRSA non-coding RNA RNA structure General Physics and Astronomy Ribonuclease III/genetics Gene Expression Regulation Bacterial General Chemistry General Biochemistry Genetics and Molecular Biology RNA Bacterial RNA Small Untranslated Methicillin-Resistant Staphylococcus aureus/genetics RNA Messenger RNA Bacterial/genetics RNA Small Untranslated/genetics |
| Zdroj: | McKellar, S W, Ivanova, I, Arede, P, Zapf, R L, Mercier, N, Chu, L-C, Mediati, D G, Pickering, A C, Briaud, P, Foster, R G, Kudla, G, Ross Fitzgerald, J, Caldelari, I, Carroll, R K, Tree, J J & Granneman, S 2022, ' RNase III CLASH in MRSA uncovers sRNA regulatory networks coupling metabolism to toxin expression ', Nature Communications, vol. 13, no. 1, 3560, pp. 1-20 . https://doi.org/10.1038/s41467-022-31173-y |
| DOI: | 10.5281/zenodo.6557838 |
| Popis: | Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial pathogen responsible for significant human morbidity and mortality. Post-transcriptional regulation by small RNAs (sRNAs) has emerged as an important mechanism for controlling virulence. However, the functionality of the majority of sRNAs during infection is unknown. To address this, we performed UV cross-linking, ligation, and sequencing of hybrids (CLASH) in MRSA to identify sRNA-RNA interactions under conditions that mimic the host environment. Using a double-stranded endoribonuclease III as bait, we uncovered hundreds of novel sRNA-RNA pairs. Strikingly, our results suggest that the production of small membrane-permeabilizing toxins is under extensive sRNA-mediated regulation and that their expression is intimately connected to metabolism. Additionally, we also uncover an sRNA sponging interaction between RsaE and RsaI. Taken together, we present a comprehensive analysis of sRNA-target interactions in MRSA and provide details on how these contribute to the control of virulence in response to changes in metabolism. |
| Databáze: | OpenAIRE |
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