High resolution DNA fingerprinting by AFLP to study the genetic variation amongOesophagostomum bifurcum(Nematoda) from human and non-human primates from Ghana
| Autor: | V. Asigri, J. M. De Gruijter, Robin B. Gasser, Anton M. Polderman, Lenie Dijkshoorn |
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| Rok vydání: | 2004 |
| Předmět: |
Population
Ghana Polymerase Chain Reaction Patas monkey Genetic variation Animals Cluster Analysis Humans Genetic variability education Genetics education.field_of_study Genetic diversity biology Primate Diseases Genetic Variation DNA Restriction Enzymes Haplorhini DNA Helminth biology.organism_classification DNA Fingerprinting Papio anubis RAPD Oesophagostomum Infectious Diseases DNA profiling Animal Science and Zoology Parasitology Amplified fragment length polymorphism Oesophagostomiasis Polymorphism Restriction Fragment Length |
| Zdroj: | Parasitology. 130:229-237 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182004006249 |
| Popis: | An AFLP approach was established to investigate genetic diversity withinOesophagostomum bifurcum(order Strongylida) from human and non-human primates. Evaluation of different combinations of restriction enzymes (n=8) and primers (n=29) demonstrated that the use ofHindIII/BglII digested templates and primers with the selective nucleotides +AG/+AC, respectively, was the most effective for the analysis ofO. bifurcumDNA. A total of 63O. bifurcumadults from human, Patas monkey, Mona monkey and Olive baboon hosts from different geographical regions in Ghana were subjected to analysis using this method. Cluster analysis revealed 4 genetically distinct groups, namelyO. bifurcumfrom the Patas monkey (I), from the Mona monkey (II), from humans (III) and from the Olive baboon (IV). These findings were concordant with those achieved previously using RAPD analysis and supports population genetic substructuring withinO. bifurcumaccording to host species. The results demonstrated the effectiveness of the present AFLP method for establishing genetic variation withinO. bifurcum, and indicates its applicability to other parasitic nematodes of human and/or veterinary health importance. |
| Databáze: | OpenAIRE |
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