Global microRNA profiles and signaling pathways in the development of cardiac hypertrophy
| Autor: | R. Hu, Huijuan Feng, M.J. Zhou, Yungang Sun, Wei Ouyang, Jialang Xian, Liu-hua Huang, C.F. Jing, J.H. Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Heart disease Physiology Immunology Biophysics Down-Regulation Ocean Engineering Cardiomegaly Constriction Pathologic Biology Biochemistry Muscle hypertrophy Rats Sprague-Dawley Downregulation and upregulation Internal medicine microRNA medicine Myocyte Animals Myocytes Cardiac General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Aorta lcsh:R5-920 Signaling pathway General Neuroscience Computational Biology Biomedical Sciences Cell Biology General Medicine Biomarker medicine.disease Prognosis Up-Regulation Disease Models Animal MicroRNAs Cardiac hypertrophy Endocrinology lcsh:Biology (General) Heart failure Cancer research Biomarker (medicine) Signal transduction lcsh:Medicine (General) Algorithms Biomarkers Signal Transduction |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Volume: 47, Issue: 5, Pages: 361-368, Published: 11 APR 2014 Brazilian Journal of Medical and Biological Research, Vol 47, Iss 5, Pp 361-368 (2014) Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research v.47 n.5 2014 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| Popis: | Hypertrophy is a major predictor of progressive heart disease and has an adverse prognosis. MicroRNAs (miRNAs) that accumulate during the course of cardiac hypertrophy may participate in the process. However, the nature of any interaction between a hypertrophy-specific signaling pathway and aberrant expression of miRNAs remains unclear. In this study, Spague Dawley male rats were treated with transverse aortic constriction (TAC) surgery to mimic pathological hypertrophy. Hearts were isolated from TAC and sham operated rats (n=5 for each group at 5, 10, 15, and 20 days after surgery) for miRNA microarray assay. The miRNAs dysexpressed during hypertrophy were further analyzed using a combination of bioinformatics algorithms in order to predict possible targets. Increased expression of the target genes identified in diverse signaling pathways was also analyzed. Two sets of miRNAs were identified, showing different expression patterns during hypertrophy. Bioinformatics analysis suggested the miRNAs may regulate multiple hypertrophy-specific signaling pathways by targeting the member genes and the interaction of miRNA and mRNA might form a network that leads to cardiac hypertrophy. In addition, the multifold changes in several miRNAs suggested that upregulation of rno-miR-331*, rno-miR-3596b, rno-miR-3557-5p and downregulation of rno-miR-10a, miR-221, miR-190, miR-451 could be seen as biomarkers of prognosis in clinical therapy of heart failure. This study described, for the first time, a potential mechanism of cardiac hypertrophy involving multiple signaling pathways that control up- and downregulation of miRNAs. It represents a first step in the systematic discovery of miRNA function in cardiovascular hypertrophy. |
| Databáze: | OpenAIRE |
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