Molecular monitoring of Neoseiulus californicus released from sheltered slow-release sachets for spider mite control in a Japanese pear greenhouse
| Autor: | Shoji Sonoda, Masatoshi Toyama, Ryuji Uesugi, Kotaro Mori, Yuya Mikawa, Mineaki Aizawa, Masahiro Osakabe |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Phytoseiidae Biology 01 natural sciences 030308 mycology & parasitology Pyrus 03 medical and health sciences Japan Spider mite Mite Animals Acari Pest Control Biological Predator 0303 health sciences Spider PEAR Tick Control Ecology fungi General Medicine biology.organism_classification 010602 entomology Horticulture Animal ecology Predatory Behavior Insect Science Tetranychidae |
| Zdroj: | SC10202003030037 NARO成果DBa C10202001280007_9276 |
| ISSN: | 1572-9702 0168-8162 |
| DOI: | 10.1007/s10493-019-00463-4 |
| Popis: | A novel system for spider mite control was developed with a slow-release sachet containing Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) protected by a waterproof shelter. Monitoring the efficacy of the predator release system for spider mite control at a Japanese pear greenhouse requires discrimination of N. californicus from other indigenous phytoseiid mite species inhabiting the study site and subsequent identification of the released N. californicus. The report of our earlier study described a PCR-based method for discrimination of N. californicus species. For the present study, we first examined phytoseiid mite species composition in the greenhouse. Subsequently, we developed microsatellite markers to identify the released N. californicus. Finally, we installed the predator release system in the greenhouse and conducted a population survey of phytoseiid and spider mites. Results demonstrated that approximately 1 month is necessary for distribution of the released N. californicus on the leaves. |
| Databáze: | OpenAIRE |
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