Preterm Birth Impedes Structural and Functional Development of Cerebellar Purkinje Cells in the Developing Baboon Cerebellum
| Autor: | Tara Barron, Jun Hee Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
NICU
Cerebellum cerebellum Purkinje cell fetal development non-human primate Biology Article lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine biology.animal medicine Extreme Preterm Birth Patch clamp lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry 030304 developmental biology 0303 health sciences Fetus baboon General Neuroscience preterm birth electrophysiology Electrophysiology medicine.anatomical_structure nervous system Cerebellar cortex Dendrite extension Neuroscience 030217 neurology & neurosurgery Baboon |
| Zdroj: | Brain Sciences, Vol 10, Iss 897, p 897 (2020) Brain Sciences Volume 10 Issue 12 |
| ISSN: | 2076-3425 |
| Popis: | Human cerebellar development occurs late in gestation and is hindered by preterm birth. The fetal development of Purkinje cells, the primary output cells of the cerebellar cortex, is crucial for the structure and function of the cerebellum. However, morphological and electrophysiological features in Purkinje cells at different gestational ages, and the effects of neonatal intensive care unit (NICU) experience on cerebellar development are unexplored. Utilizing non-human primate baboon cerebellum, we investigated Purkinje cell development during the last trimester of pregnancy and the effect of NICU experience following premature birth on developmental features of Purkinje cells. Immunostaining and whole-cell patch clamp recordings of Purkinje cells in the baboon cerebellum at different gestational ages revealed that molecular layer width, driven by Purkinje dendrite extension, drastically increased and refinement of action potential waveform properties occurred throughout the last trimester of pregnancy. Preterm birth followed by NICU experience for 2 weeks impeded development of Purkinje cells, including action potential waveform properties, synaptic input, and dendrite extension compared with age-matched controls. In addition, these alterations impact Purkinje cell output, reducing the spontaneous firing frequency in deep cerebellar nucleus (DCN) neurons. Taken together, primate cerebellum undergoes developmental refinements during late gestation, and NICU experience following preterm birth alters morphological and physiological features in the cerebellum that can lead to functional deficits.Summary StatementBaboon cerebellum undergoes developmental refinements during late gestation, and NICU experience following preterm birth impacts cellular development in the cerebellum that can lead to functional deficits. |
| Databáze: | OpenAIRE |
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