Prediction of the profiles of the elution bands of proteins in preparative liquid chromatography

Autor:	Georges Guiochon, Anita M. Katti, Jun-Xiong Huang
Rok vydání:	1990
Předmět:	High concentration Aqueous solution Chromatography Isocratic elution Elution Chemistry Analytical chemistry Albumin Bioengineering Applied Microbiology and Biotechnology Phase (matter) Conalbumin Anion exchanger Biotechnology
Zdroj:	Biotechnology and bioengineering. 36(3)
ISSN:	0006-3592
Popis:	A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isocratic elution bands of chicken albumin and conalbumin on a weak anion exchanger in aqueous buffered solutions. Satisfactory agreement is observed between the experimental elution profiles of large-sample-size bands of chicken albumin and conalbumin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a41379e575e1f60a500c8d4a868f274 https://pubmed.ncbi.nlm.nih.gov/18595080 Zobrazit plný text záznamu Plný text