The neurosteroid pregnenolone sulphate enhances NMDA-induced phasic firing of vasopressin neurones in the rat supraoptic nucleus
| Autor: | C.M. Richardson, J. B. Wakerley |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Vasopressin
medicine.medical_specialty N-Methylaspartate Time Factors Neuroactive steroid Vasopressins Hypothalamus Neuropeptide In Vitro Techniques Biology Supraoptic nucleus chemistry.chemical_compound Internal medicine medicine Animals Picrotoxin Rats Wistar Evoked Potentials Neurons Dehydroepiandrosterone Sulfate musculoskeletal neural and ocular physiology General Neuroscience Drug Synergism Rats Endocrinology nervous system chemistry Pregnenolone NMDA receptor Female Supraoptic Nucleus medicine.drug |
| Zdroj: | Neuroscience Letters. 226:123-126 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(97)00260-7 |
| Popis: | The effect of the neurosteroid pregnenolone sulphate (PS) on N -methyl- d -aspartate (NMDA)-induced phasic firing of supraoptic vasopressin (VP) neurones was studied in rat hypothalamic slices in vitro. In VP neurones which were induced to fire phasically by continuous perifusion with NMDA (9–30 μ M), addition of 100 μ M PS to the incubation medium significantly increased overall spike frequency, with a rise in both proportion of time active and intraburst firing rate. A similar effect was seen during picrotoxin block of GABAergic transmission. No significant change in NMDA-induced phasic firing was observed with 100 μ M dehydroepiandrosterone sulphate. VP neurones became silent in the absence of NMDA, and under these conditions PS had no effect. In conclusion, PS increases NMDA-induced phasic firing in VP neurones, providing a mechanism whereby this neurosteroid may participate in the regulation of VP secretion. |
| Databáze: | OpenAIRE |
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