Preparation, physicochemical characterization, and anti-proliferative properties of Lawsone-loaded solid lipid nanoparticles
| Autor: | Sattar Akbari Nakhjavani, Raheleh Farahzadi, Masoud Eskandani, Somayeh Vandghanooni, Mehdi Jaymand, Forough Rasouliyan, Morteza Eskandani |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cell Survival
Antineoplastic Agents medicine.disease_cause Biochemistry Lawsone chemistry.chemical_compound In vivo Solid lipid nanoparticle Zeta potential medicine Humans Fragmentation (cell biology) Particle Size Molecular Biology Drug Carriers Cell Death Organic Chemistry Cell Biology Cell Cycle Checkpoints body regions Comet assay Gene Expression Regulation Neoplastic Drug Liberation Kinetics chemistry A549 Cells Liposomes Nanoparticles Genotoxicity Homogenization (biology) Nuclear chemistry Naphthoquinones |
| Zdroj: | Chemistry and physics of lipids. 239 |
| ISSN: | 1873-2941 |
| Popis: | Lawsone (LWS) is a naphthoquinone-type dye with potential antitumor activity. LWS is used in cosmetics for coloring hair, skin, and nails. In this study, solid lipid nanoparticles (SLNs) containing LWS were prepared using a hot homogenization technique. Physicochemical properties of LWS-SLNs including encapsulation efficiency (EE), drug loading (DL), size, zeta potential, homogeneity, in vitro release, and kinetics of release were determined. The potential cytotoxic properties of LWS-SLNs were investigated. Comet assay was done to assess the genotoxicity of LWS-SLNs. The scanning electron microscopy (SEM) images revealed that LWS-SLNs were spherical and homogeneously dispersed. The average diameter of free SLNs and LWS-SLNs were 97 ± 1.4 and 127 ± 3.1 nm, respectively with high EE% (95.88 ± 3.29) and a DL of 22.72 ± 1.39 mg/mL of LWS-SLNs. The plain LWS could induce growth inhibition of A549 cells with IC50 of 17.99 ± 1.11, 13.37 ± 1.22, and 9.21 ± 1.15 μg/mL after 24, 48, and 72 h, respectively, while LWS-SLNs had more cytotoxic effects after 48 h (9.81 ± 1.3 μg/mL). Comet assay represented clear fragmentation in the chromatin of the treated cells. Besides, LWS-SLNs (13.37 ± 1.22 μg/mL) induced ∼52 % apoptosis and even necrosis after 48 h. The qPCR results showed an enhanced downregulation of Bcl-2 and upregulation of Casp 9 due to the treatment of A549 cells with LSW-SLNs. In conclusion, a stable formulation of LWS-SLN was prepared with good physicochemical features and long-term biological effects that candidate it for in vivo trials. |
| Databáze: | OpenAIRE |
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