Genetic diversity of Mycobacterium tuberculosis and transmission associated with first-line drug resistance: a first analysis in Jalisco, Mexico
| Autor: | Manuel Sandoval-Díaz, Carlos A. Vazquez-Chacon, Gustavo Mora-Aguilera, Guadalupe Gonzalez-Palomar, Martín Lopez-Rodriguez, Gladys Lopez-Avalos, Ulises Miranda-Hernández, Ikuri Alvarez-Maya, Juan Carlos Villanueva-Arias, Juan Antonio González-Barrios |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Genotyping Techniques 030106 microbiology Immunology Population Antitubercular Agents Drug resistance Microbial Sensitivity Tests Gene mutation Biology Microbiology Mycobacterium tuberculosis 03 medical and health sciences Bacterial Proteins Tuberculosis Multidrug-Resistant Isoniazid Immunology and Allergy Humans Genetic variability education Genotyping Mexico Phylogeny education.field_of_study INHA Genetic Variation DNA-Directed RNA Polymerases rpoB biology.organism_classification Catalase Virology Mutation Rifampin Oxidoreductases |
| Zdroj: | Journal of global antimicrobial resistance. 11 |
| ISSN: | 2213-7173 |
| Popis: | Objectives The objectives of this study were to analyse the frequency of gene mutations associated with antitubercular drug resistance in clinical samples from the population of Jalisco State (Mexico) and to evaluate the genetic variability of Mycobacterium tuberculosis and multidrug-resistant (MDR) M. tuberculosis strains to describe the frequency of various families. Methods Clinical isolates of M. tuberculosis obtained from Jalisco State were analysed. Isolates were subjected to drug susceptibility testing, and mutations were characterised by sequencing, followed by genotyping using spoligotyping and mycobacterial interspersed repetitive units–variable-number of tandem repeats (MIRU-VNTR). Moreover, the prevalence of mutations was analysed by phylogenetic lineages. Results Resistant strains were analysed by sequencing of katG , inhA and rpoB genes to determine the presence of mutations associated with isoniazid and rifampicin resistance. In MDR, monoresistant and polyresistant isolates, mutations were found in 17 (54.84%) of 31 strains. Spoligotyping identified six different strain lineages [T1 (25.40%), H3 (7.94%), MANU (4.76%), X1 (3.17%), EAI5 (1.59%) and LAM1 (1.59%)], with the remaining strains identified as orphans. In additional tree-based identification, a dendrogram of spoligotype patterns generated five different similarity clusters. When combining 24-loci MIRU-VNTR and spoligotyping approaches, the results shows that there is no cluster formation, indicating low transmission of the samples. Conclusions This study using spoligotyping and MIRU-VNTR showed that the analysed strains were not related to each other since no two identical strains were found. Families with the highest prevalence in the study were orphans followed by T family. |
| Databáze: | OpenAIRE |
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