Comparison of three artificial digestion methods for detection of non-encapsulated Trichinella pseudospiralis larvae in pork
| Autor: | Karsten Nöckler, J. van der Giessen, S. Reckinger, Charlotte Maddox-Hyttel, P. Boireau, Isabelle Vallée, I. Szabo, Edoardo Pozio |
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| Přispěvatelé: | Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), National Veterinary Institute, Technical University of Denmark [Lyngby] (DTU), Istituto Superiore di Sanità, National Institute for Public Health and the Environment [Bilthoven] (RIVM), Unité mixte de recherche biologie moléculaire et immunologie parasitaires et fongiques, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) |
| Rok vydání: | 2009 |
| Předmět: |
Quality Control
Pathology medicine.medical_specialty MAGNETIC STIRRER METHOD Meat TRICHOMATIC 35 (R) Swine Trichinella 030231 tropical medicine Trichinella pseudospiralis Sensitivity and Specificity 030308 mycology & parasitology Artificial digestion 03 medical and health sciences 0302 clinical medicine Food Parasitology medicine Animals Food science STOMACHER METHOD Minced pork 0303 health sciences Larva [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health General Veterinary biology Clinical Laboratory Techniques fungi Reproducibility of Results General Medicine biology.organism_classification Laboratory results TRICHINELLA PSEUDOSPIRALIS Consumer Product Safety Nematode larvae Parasitology Digestion human activities |
| Zdroj: | Veterinary Parasitology 12. International Conference on Trichinellosis 12. International Conference on Trichinellosis, Sep 2007, Plitvice Lakes National Park, Croatia. ⟨10.1016/j.vetpar.2008.10.075⟩ |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2008.10.075 |
| Popis: | International audience; In a ring trial involving five laboratories (A, B, C, D, and E), three different methods of artificial digestion were compared for the detection of non-encapsulated Trichinella pseudospiralis larvae in minced meat. Each sample panel consisted often 1 g minced pork samples. All samples in each panel were derived from a bulk meat preparation with a nominal value of either 7 or 17 larvae per g (Ipg). Samples were tested for the number of muscle larvae using the magnetic stirrer method (labs A, B, and E), stomacher method (lab B), and Trichomatic 35 (R) (labs C and D). T. pseudospiralis larvae were found in all 120 samples tested. For samples with 7 Ipg, larval recoveries were significantly higher using the stomacher method versus the magnetic stirrer method, but there were no significant differences for samples with 17 Ipg. In comparing laboratory results irrespective of the method used, lab B detected a significantly higher number of larvae than lab E for samples with 7 Ipg, and lab E detected significantly less larvae than labs A, B, and D in samples with 17 Ipg. The lowest overall variation for quantitative results (i.e. larval recoveries which were outside the tolerance range) was achieved by using the magnetic stirrer method (22%), followed by the stomacher method (25%), and Trichomatic 35 (R) (30%). Results revealed that T. pseudospiralis larvae in samples with a nominal value of 7 and 17 Ipg can be detected by all three methods of artificial digestion. |
| Databáze: | OpenAIRE |
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