Global analysis of genes differentially expressed in branching and non-branching regions of the mouse embryonic lung

Autor: Jun Qian, Konstantin I. Izvolsky, Wellington V. Cardoso, Jining Lu
Rok vydání: 2004
Předmět:
DNA
Complementary
Microarrays
Receptors
Retinoic Acid
Ubiquitin-Protein Ligases
Retinoic acid
Respiratory tract
WWP2
Gestational Age
Tretinoin
In situ hybridization
Biology
Fibroblast growth factor
03 medical and health sciences
chemistry.chemical_compound
Mice
TGF beta
Transforming Growth Factor beta
Branching morphogenesis
Animals
Gene
Molecular Biology
Lung
In Situ Hybridization
030304 developmental biology
Body Patterning
Oligonucleotide Array Sequence Analysis
0303 health sciences
Extracellular Matrix Proteins
Base Sequence
Gene Expression Profiling
030302 biochemistry & molecular biology
Ubiquitination
Wnt signaling pathway
Gene Expression Regulation
Developmental
Cell Biology
Molecular biology
Tgfbi
Wwp2
chemistry
RNA splicing
Lung development
RNA
DNA microarray
Growth factors
Developmental Biology
Zdroj: Developmental biology. 273(2)
ISSN: 0012-1606
Popis: During development, the proximal and distal regions of respiratory tract undergo distinct processes that ultimately give rise to conducting airways and alveoli. To gain insights into the genetic pathways differentially activated in these regions when branching morphogenesis is initiating, we characterized their transcriptional profiles in murine rudiments isolated at embryonic (E) day 11.5. By using oligonucleotide microarrays, we identified 83 and 128 genes preferentially expressed in branching and non-branching regions, respectively. The majority of these genes (85%) had not been previously described in the lung, or in other organs. We report restricted expression patterns of 22 of these genes were by in situ hybridization. Among them in the lung potential components of the Wnt, TGF beta, FGF and retinoid pathways identified in other systems, and uncharacterized genes, such as translocases, small GTPases and splicing factors. In addition, we provide a more detailed analysis of the expression pattern and regulation of a representative gene from the distal (transforming growth factor, beta induced) and proximal (WW domain-containing protein 2) regions. Our data suggest that these genes may regulate focal developmental events specific of each of these regions during respiratory tract formation.
Databáze: OpenAIRE
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