TRPA1 and TRPV4 Activation in Human Odontoblasts Stimulates ATP Release
| Autor: | L. Di Silvio, M. Yazdi, A.K. Duggal, Obi Egbuniwe, Andrew D. Grant, S. Grover, A. Mavroudis, Tara Renton |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
TRPV4
Agonist Pathology medicine.medical_specialty medicine.drug_class Cell Culture Techniques TRPV1 TRPM Cation Channels TRPV Cation Channels Nerve Tissue Proteins Pyrimidinones Cell Line chemistry.chemical_compound Transient receptor potential channel Adenosine Triphosphate Transient Receptor Potential Channels stomatognathic system Isothiocyanates Leucine medicine TRPM8 Humans Calcium Signaling Acrolein TRPA1 Cation Channel General Dentistry Dental Pulp Sulfonamides Odontoblasts Voltage-dependent calcium channel Chemistry Icilin Nociceptors Research Reports Cell biology Purines Cell culture Culture Media Conditioned Sensory System Agents Acetanilides Calcium Channels Capsaicin |
| Zdroj: | Journal of Dental Research. 93:911-917 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | The mechanism of pain in dentine hypersensitivity is poorly understood but proposed to result from the activation of dental sensory neurons in response to dentinal fluid movements. Odontoblasts have been suggested to contribute to thermal and mechanosensation in the tooth via expression of transient receptor potential (TRP) channels. However, a mechanism by which odontoblasts could modulate neuronal activity has not been demonstrated. In this study, we investigated functional TRP channel expression in human odontoblast-like cells and measured ATP release in response to TRP channel activation. Human immortalized dental pulp cells were driven toward an odontoblast phenotype by culture in conditioned media. Functional expression of TRP channels was determined with reverse transcription polymerase chain reaction and ratiometric calcium imaging with Fura-2. ATP release was measured using a luciferin-luciferase assay. Expression of mRNA for TRPA1, TRPV1, and TRPV4 but not TRPM8 was detected in odontoblasts by reverse transcription polymerase chain reaction. Expression of TRPV4 protein was detected by Western blotting and immunocytochemistry. The TRPA1 agonists allyl isothiocyanate and cinnamaldehyde and the TRPV4 agonist GSK1016790A caused a concentration-dependent increase in intracellular Ca2+ concentration that was inhibited by the selective antagonists HC030031, AP18, and HC067047, respectively. In contrast, exposure to the TRPV1 agonist capsaicin or the TRPM8 agonist icilin had no effect on intracellular Ca2+ concentration. Treatment with allyl isothiocyanate, cinnamaldehyde, or GSK1016790A caused an increase in ATP concentration in culture medium that was abolished by preincubation with TRP channel antagonists. These data demonstrate that activation of TRPA1 and TRPV4 channels in human odontoblast-like cells can stimulate ATP release. We were unable to confirm the presence of thermosensitive TRPV1 and TRPM8 that has previously been reported in odontoblasts. |
| Databáze: | OpenAIRE |
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