Hyper-expression of Osteocalcin mRNA in Odontoblasts of Hyp Mice
| Autor: | Tomoyuki Onishi, Takashi Ooshima, R. Okawa, Tomohiko Ogawa, Tomonori Hoshino, T. Hayashibara |
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| Rok vydání: | 2005 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Osteocalcin Gene Expression Matrix (biology) Mice 03 medical and health sciences 0302 clinical medicine stomatognathic system Internal medicine Dentin medicine Animals Humans RNA Messenger General Dentistry Hypophosphatemia Familial In Situ Hybridization Messenger RNA Odontoblasts biology Reverse Transcriptase Polymerase Chain Reaction Chemistry Tooth Germ 030206 dentistry Dentinogenesis Mice Mutant Strains Mice Inbred C57BL stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Endocrinology Odontoblast Models Animal biology.protein Osteonectin Tooth Calcification Type I collagen |
| Zdroj: | Journal of Dental Research. 84:84-88 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | The Hyp mouse is a murine homologue of human X-linked hypophosphatemia that displays hypo-mineralization in bone and dentin. In this study, we tested the hypothesis that the defect in Hyp mice leads to alterations in the expression of dentin matrix proteins that may be associated with the hypo-mineralization changes in the tissues. Quantitative RT-PCR analyses showed that expression of the osteocalcin gene in Hyp mice tooth germ samples was significantly higher than in wild-type mice, whereas the gene expressions of osteonectin, osteopontn, dentin matrix protein 1, and type I collagen in both types of mice were similar. Further, cultured Hyp mice tooth germ samples exhibited a higher expression of the osteocalcin gene than did those from wild-type mice, which was in accord with the results of our in vivo analysis. These findings suggest that osteocalcin mRNA is highly expressed in Hyp mice odontoblasts and may be associated with dentin hypo-mineralization. |
| Databáze: | OpenAIRE |
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