In vitro Antioxidant and Anti-obesity Activities of Freeze-dried Canarium sp., Averrhoa bilimbi L. and Malus domestica
| Autor: | Dea Anindya, Wahyu Widowati, Susi Endrini, Intan Meila Tria Lestari, Engkay Abu Bakar, Fahrul Rozy, Sri Puji Utami, Satrio Wibowo, Said Nafik, Seila Arumwardana, Hanna Sari Widya Kusuma, Hayatun Nufus, Ervi Afifah, Fathonah Fatimatuzahra Said, Dwi Davidson Rihibiha |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
food.ingredient Averrhoa bilimbi 030309 nutrition & dietetics DPPH medicine.medical_treatment Flavonoid Dietary lipid Medicine (miscellaneous) General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound food medicine Food science 030304 developmental biology chemistry.chemical_classification 0303 health sciences lcsh:R5-920 ABTS biology Canarium biology.organism_classification Hydroxycitric acid chemistry lcsh:Medicine (General) |
| Zdroj: | Indonesian Biomedical Journal, Vol 11, Iss 3, Pp 320-6 (2019) |
| ISSN: | 2355-9179 2085-3297 |
| Popis: | BACKGROUND: The number of obesity cases is still increasing worldwide and has reached an epidemic scale. Plants are known to have a protection role in the development of obesity, however their antioxidant and anti-obesity activities have not widely known. This study was conducted to assess the in vitro antioxidant and anti-obesity activities of different types of freeze-dried fruits cultivated in Indonesia, especially Canarium sp., Averrhoa bilimbi L. and Malus domestica . METHODS: Total phenolic content of freeze-dried fruits was identified by Folin-Ciocalteu method, while the total flavonoid content was measured by aluminium chloride colorimetric assay. To assessed the antioxidant activity, 2,2-diphenyl 1-picrylhydrazyl (DPPH) scavenging activity assay and 2,2’-azino-bis (3-ethylbenzothiazoline6-sulphonic acid) (ABTS) reducing activity assay were performed. The α-amylase and lipase inhibitory activity assay were performed to assess the anti-obesity activity. For comparison, hydroxycitric acid (HCA) compound was also assessed with DPPH, ABTS, α-amylase and lipase assays. RESULTS: A. bilimbi had the highest total phenol content (6.35 µg GAE/mg), meanwhile M. domestica had the highest total flavonoid content (2.06 µg QE/mg). A. bilimbi also showed the highest antioxidant activity both in DPPH and ABTS assay, with inhibitory concentration (IC50)=279.99 µg/mL and 631.78 µg/mL, respectively. The freeze-dried M. domestica had the highest anti-α-amylase activity (IC50=258.85 µg/mL), while Canarium sp. had the highest anti-lipase activity (IC50=118.66 µg/mL). CONCLUSION: Freeze-dried fruits demonstrate in vitro benefits toward obesity. A. bilimbi has potent antioxidant activity and is beneficial against obesity-related adverse health effect by relieving oxidative stress. M. domestica and Canarium sp. hamper the fat accumulation by reducing the carbohydrate absorption and dietary lipid. KEYWORDS: antioxidant, anti-obesity, Canarium sp., Averrhoa bilimbi L., Malus domestica , hydroxycitric acid |
| Databáze: | OpenAIRE |
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