Analysis of PPARγ Signaling Activity in Psoriasis
| Autor: | Elena Denisova, Anastasia P. Nesterova, A. L. Piruzyan, Vladimir Sobolev, Evgenia Dvoriankova, I.M. Korsunskaya, A. G. Soboleva, Alexandre Mezentsev, Olga Melnichenko |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
signaling pathway
QH301-705.5 Catalysis Proinflammatory cytokine Inorganic Chemistry RAR-related orphan receptor gamma Psoriasis Medicine Biology (General) Physical and Theoretical Chemistry STAT3 QD1-999 Molecular Biology Transcription factor Spectroscopy biology business.industry Organic Chemistry FOXP3 General Medicine psoriasis medicine.disease peroxisome proliferator-activated receptor gamma (PPARγ) Computer Science Applications Chemistry Nuclear receptor immunohistochemistry Cancer research biology.protein ELISA Signal transduction business real-time PCR |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 16 International Journal of Molecular Sciences, Vol 22, Iss 8603, p 8603 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22168603 |
| Popis: | In our previous work, we built the model of PPARγ dependent pathways involved in the development of the psoriatic lesions. Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor and transcription factor which regulates the expression of many proinflammatory genes. We tested the hypothesis that low levels of PPARγ expression promote the development of psoriatic lesions triggering the IL17-related signaling cascade. Skin samples of normally looking and lesional skin donated by psoriasis patients and psoriatic CD3+ Tcells samples (n = 23) and samples of healthy CD3+ T cells donated by volunteers (n = 10) were analyzed by real-time PCR, ELISA and immunohistochemistry analysis. We found that the expression of PPARγ is downregulated in human psoriatic skin and laser treatment restores the expression. The expression of IL17, STAT3, FOXP3, and RORC in psoriatic skin before and after laser treatment were correlated with PPARγ expression according to the reconstructed model of PPARγ pathway in psoriasis.In conclusion, we report that PPARγ weakens the expression of genes that contribute in the development of psoriatic lesion. Our data show that transcriptional regulation of PPARγ expression by FOSL1 and by STAT3/FOSL1 feedback loop may be central in the psoriatic skin and T-cells. |
| Databáze: | OpenAIRE |
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