Frequency-swept HMQC sequences for high-throughput NMR analysis
Autor: | Timothy D, Spitzer, Randy D, Rutkowske, George F, Dorsey |
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Rok vydání: | 2008 |
Předmět: | |
Zdroj: | Magnetic Resonance in Chemistry. 46:558-563 |
ISSN: | 1097-458X 0749-1581 |
Popis: | We describe here new versions of the DEPT phase-encoded HMQC experiment that offer robust performance and improved sensitivity. The new sequences rely on frequency-swept proton and carbon pulses to minimize signal losses from miscalibrated pulses while providing 'J compensation' to optimize the signal strength over a range of heteronuclear coupling constants. By including both proton and carbon-swept pulses, the new sequences also offer an additional signal gain of roughly 10% over well-calibrated hard-pulse experiments. The new sequences also demonstrate that one can construct a sequence that incorporates both 90 degrees and 180 degrees frequency-swept pulses. Although individual pulses in the sequence cause severe phase roll, the phase roll can be eliminated by the proper choice of pulse lengths and sweep directions. |
Databáze: | OpenAIRE |
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