Frequency-swept HMQC sequences for high-throughput NMR analysis

Autor:	Timothy D, Spitzer, Randy D, Rutkowske, George F, Dorsey
Rok vydání:	2008
Předmět:	Carbon Isotopes Reproducibility of Results Signal Processing Computer-Assisted General Materials Science General Chemistry Protons Nuclear Magnetic Resonance Biomolecular Sensitivity and Specificity Algorithms
Zdroj:	Magnetic Resonance in Chemistry. 46:558-563
ISSN:	1097-458X 0749-1581
Popis:	We describe here new versions of the DEPT phase-encoded HMQC experiment that offer robust performance and improved sensitivity. The new sequences rely on frequency-swept proton and carbon pulses to minimize signal losses from miscalibrated pulses while providing 'J compensation' to optimize the signal strength over a range of heteronuclear coupling constants. By including both proton and carbon-swept pulses, the new sequences also offer an additional signal gain of roughly 10% over well-calibrated hard-pulse experiments. The new sequences also demonstrate that one can construct a sequence that incorporates both 90 degrees and 180 degrees frequency-swept pulses. Although individual pulses in the sequence cause severe phase roll, the phase roll can be eliminated by the proper choice of pulse lengths and sweep directions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a32033a5ffa744390bec9e562dabe87 https://doi.org/10.1002/mrc.2214 Zobrazit plný text záznamu Plný text