Effect of dose and dose rate of gamma irradiation on the formation of micronuclei in bone marrow cells isolated from whole‐body‐irradiated mice

Autor: Ali Pour Khavari, Traimate Sangsuwan, Paulo Roberto D'Auria Vieira de Godoy, Ayumi Nakamura, Siamak Haghdoost
Přispěvatelé: The Wenner-Gren Institute, Stockholm University, Accueil et Recherche en Radiobiologie des Ions Accélérés (ARIA), Centre de recherche sur les Ions, les MAtériaux et la Photonique (CIMAP - UMR 6252), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
medicine.medical_specialty
Epidemiology
DNA damage
Health
Toxicology and Mutagenesis
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Bone Marrow Cells
Endogeny
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
medicine.disease_cause
Ionizing radiation
Mice
Internal medicine
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Irradiation
Micronuclei
Chromosome-Defective
Genetics (clinical)
chemistry.chemical_classification
Reactive oxygen species
Micronucleus Tests
Chemistry
Dose-Response Relationship
Radiation
Mice
Inbred C57BL
Endocrinology
medicine.anatomical_structure
Gamma Rays
Micronucleus test
Female
Bone marrow
Whole-Body Irradiation
Oxidative stress
Zdroj: Environmental and Molecular Mutagenesis
Environmental and Molecular Mutagenesis, 2021, 62 (7), pp.422-427. ⟨10.1002/em.22453⟩
ISSN: 0893-6692
DOI: 10.1002/em.22453⟩
Popis: It is well-known that the cytotoxicity and mutagenic effects of high dose rate (HDR) ionizing radiation (IR) are increased by increasing the dose but less is known about the effects of chronic low dose rate (LDR). In vitro, we have shown that in addition to the immediate interaction of IR with DNA (the direct and indirect effects), low doses and chronic LDR exposure induce endogenous oxidative stress. During elevated oxidative stress, reactive oxygen species (ROS) react with DNA modifying its structure. Here, BL6 mice were exposed to IR at LDR and HDR and were then sacrificed 3 hours and 3 weeks after exposure to examine early and late effects of IR. The levels of micronuclei, MN, were determined in bone marrow cells. Our data indicate that the effects of 200 mGy on MN-induction are transient, but 500 and 1000 mGy (both HDR and LDR) lead to increased levels of MN up to 3 weeks after the exposure.
Databáze: OpenAIRE
Externí odkaz: