Paper-based colorimetric biosensor of blood alcohol with in-situ headspace separation of ethanol from whole blood
| Autor: | Duangjai Nacapricha, Saranya Auparakkitanon, Thitaporn Sonsa-ard, Yanisa Thepchuay, Jirayu Sitanurak, Nuanlaor Ratanawimarnwong |
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| Rok vydání: | 2020 |
| Předmět: |
Paper
Biosensing Techniques 02 engineering and technology 01 natural sciences Biochemistry Horseradish peroxidase Armoracia Analytical Chemistry Mice chemistry.chemical_compound Animals Environmental Chemistry Benzothiazoles Horseradish Peroxidase Spectroscopy Whole blood Chromatography Ethanol biology Filter paper Chemistry Chromogenic 010401 analytical chemistry Reproducibility of Results 021001 nanoscience & nanotechnology 0104 chemical sciences Alcohol oxidase Alcohol Oxidoreductases Reagent biology.protein Colorimetry Indicators and Reagents Sulfonic Acids 0210 nano-technology Biosensor |
| Zdroj: | Analytica Chimica Acta. 1103:115-121 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2019.12.043 |
| Popis: | This work presents a novel development that exploits the concept of in-situ gas-separation together with a specific enzymatic colorimetric detection to produce a portable biosensor called “Blood Alcohol Micro-pad” for direct quantitation of ethanol in whole blood. The thin square device (25 mm × 25 mm × 1.8 mm) comprises two layers of patterned filter paper held together with a double-sided mounting tape with an 8-mm circular hole (the headspace). In operation, the reagent is deposited on one layer and covered with sticky tape. Then 8 μL of a blood sample is dispensed onto the opposite layer and covered with sticky tape. Diffusion of ethanol across the 1.6 mm narrow headspace permits selective detection of ethanol by the enzymatic reagents deposited on the opposite layer. This reagent zone contains alcohol oxidase, horseradish peroxidase and 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, as the chromogenic reagent. The color intensity, measured from the recorded digital image, resulting from the enzymatic assay of ethanol, correlates with the concentration of blood alcohol. The results obtained with spiked mice and sheep blood samples, using an external calibration in the range of 1–120 mg dL−1ethanol, gave recoveries of 93.2–104.4% (n = 12). The “Blood Alcohol Micro-pad” gave good precision with %RSD |
| Databáze: | OpenAIRE |
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