An Amino-Terminal Polo Kinase Interaction Motif Acts in the Regulation of Centrosome Formation and Reveals a Novel Function for centrosomin (cnn) in Drosophila
| Autor: | Melissa A. S. Phelps, Thomas C. Kaufman, Robert C. Eisman |
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| Rok vydání: | 2015 |
| Předmět: |
animal structures
Embryo Nonmammalian Embryonic Development Protein Serine-Threonine Kinases Investigations Microtubules Exon Meiosis Microtubule Genetics Animals Drosophila Proteins Centrosome Homeodomain Proteins biology Polo kinase Gene Expression Regulation Developmental Exons biology.organism_classification Cell biology enzymes and coenzymes (carbohydrates) Drosophila melanogaster Phosphorylation biological phenomena cell phenomena and immunity Drosophila Protein |
| Zdroj: | Genetics. 201:685-706 |
| ISSN: | 1943-2631 |
| Popis: | The formation of the pericentriolar matrix (PCM) and a fully functional centrosome in syncytial Drosophila melanogaster embryos requires the rapid transport of Cnn during initiation of the centrosome replication cycle. We show a Cnn and Polo kinase interaction is apparently required during embryogenesis and involves the exon 1A-initiating coding exon, suggesting a subset of Cnn splice variants is regulated by Polo kinase. During PCM formation exon 1A Cnn-Long Form proteins likely bind Polo kinase before phosphorylation by Polo for Cnn transport to the centrosome. Loss of either of these interactions in a portion of the total Cnn protein pool is sufficient to remove native Cnn from the pool, thereby altering the normal localization dynamics of Cnn to the PCM. Additionally, Cnn-Short Form proteins are required for polar body formation, a process known to require Polo kinase after the completion of meiosis. Exon 1A Cnn-LF and Cnn-SF proteins, in conjunction with Polo kinase, are required at the completion of meiosis and for the formation of functional centrosomes during early embryogenesis. |
| Databáze: | OpenAIRE |
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