The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase
| Autor: | John H. Willis, Jacque-Lynne Johnson, Sarah Glaser, Matthias Peter, Bruce Bowerman, Andrew Willems, Lionel Pintard, Thimo Kurz, Paul E. Mains, Martin Srayko, Mike Tyers |
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| Rok vydání: | 2003 |
| Předmět: |
Macromolecular Substances
Ubiquitin-Protein Ligases RBX1 Amino Acid Motifs Molecular Sequence Data Mitosis Cell Cycle Proteins Katanin Microtubules Substrate Specificity Ligases Ubiquitin Skp1 Animals Amino Acid Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins Alleles Adaptor Proteins Signal Transducing Adenosine Triphosphatases Multidisciplinary biology Cullin Proteins Ubiquitin ligase Meiosis Protein Subunits Biochemistry Proteasome Mutation biology.protein RNA Interference Carrier Proteins Cullin Protein Binding |
| Zdroj: | Nature. 425:311-316 |
| ISSN: | 1476-4687 0028-0836 |
| Popis: | Many biological processes, such as development and cell cycle progression are tightly controlled by selective ubiquitin-dependent degradation of key substrates. In this pathway, the E3-ligase recognizes the substrate and targets it for degradation by the 26S proteasome. The SCF (Skp1–Cul1–F-box) and ECS (Elongin C–Cul2–SOCS box) complexes are two well-defined cullin-based E3-ligases1, 2, 3. The cullin subunits serve a scaffolding function and interact through their C terminus with the RING-finger-containing protein Hrt1/Roc1/Rbx1, and through their N terminus with Skp1 or Elongin C, respectively. In Caenorhabditis elegans, the ubiquitin-ligase activity of the CUL-3 complex is required for degradation of the microtubule-severing protein MEI-1/katanin at the meiosis-to-mitosis transition4. However, the molecular composition of this cullin-based E3-ligase is not known. Here we identified the BTB-containing protein MEL-26 as a component required for degradation of MEI-1 in vivo. Importantly, MEL-26 specifically interacts with CUL-3 and MEI-1 in vivo and in vitro, and displays properties of a substrate-specific adaptor. Our results suggest that BTB-containing proteins may generally function as substrate-specific adaptors in Cul3-based E3-ubiquitin ligases. |
| Databáze: | OpenAIRE |
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