Evaluation of prostaglandin analogue effects on corneal keratocyte density using scanning laser confocal microscopy
| Autor: | Sylvia Marchi, Chiara Bergonzi, Mirella Blini, Andrea Giani, Saverio Luccarelli, Giovanni Staurenghi |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty genetic structures medicine.drug_class Corneal Stroma Adrenergic beta-Antagonists Prostaglandin Glaucoma Cell Count law.invention Extracellular matrix chemistry.chemical_compound law Confocal microscopy Ophthalmology medicine Humans Aged Microscopy Confocal business.industry Fibroblasts Middle Aged medicine.disease Laser eye diseases Extracellular Matrix Prostaglandin analog medicine.anatomical_structure chemistry Case-Control Studies Prostaglandins F Synthetic Female sense organs Corneal keratocyte Prostaglandin analogue business |
| Zdroj: | Journal of glaucoma. 19(9) |
| ISSN: | 1536-481X |
| Popis: | To determine if treatment with prostaglandin (PG) analogues affects keratocyte density as an indirect measure of extracellular matrix in the corneal stroma.In this case control study, 129 eyes from 68 patients were examined: 52 eyes had only PG analogue therapy for at least 3 years, 37 eyes had only β-adrenergic blocker therapy for at least 3 years, and 40 control eyes were without therapy. Scanning laser confocal microscopy was carried out, and each corneal stroma was subdivided into the anterior, midstroma, and posterior stroma. Keratocyte density was determined from the images by manual counting.Keratocyte densities in the entire stroma and in 2 stromal layers were significantly higher in patients with PG analogue therapy compared with control patients (entire stroma, P0.001; anterior stroma, P=0.001; posterior stroma, P=0.016). Keratocyte density in the PG analogue therapy patients was also greater compared with the β-adrenergic blocker patients (entire stroma, P0.001; anterior stroma, P0.001; mid-stroma P=0.023, posterior stroma P=0.007). There were no significant differences between the control and β-adrenergic blocker groups. Overall, the density increase for the PG analogue group was greatest in the anterior stroma.PG analogue therapy increased the keratocyte density in each layer of corneal stroma, but more significantly in the anterior stroma than in the midstroma or the posterior stroma. The increased keratocyte density might be the result of diminished extracellular matrix, possibly owing to the known activation of matrix metalloproteinases and inhibition of tissue inhibitors of the metalloproteinases. |
| Databáze: | OpenAIRE |
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