Interferon-gamma and tumor necrosis factor-alpha sensitize primarily resistant human endometrial stromal cells to Fas-mediated apoptosis
| Autor: | Peter Licht, Margarita Deperschmidt, Diethelm Wallwiener, Stefanie Krenzer, Sebastian Wesselborg, Herbert Fluhr, Björn Stork, Gerburg M. Stein, Marek Zygmunt |
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| Rok vydání: | 2007 |
| Předmět: |
Programmed cell death
Time Factors Mitomycin CASP8 and FADD-Like Apoptosis Regulating Protein Cell Culture Techniques Caspase 3 Apoptosis Cell Separation Biology Caspase 8 Chorionic Gonadotropin Fas ligand CFLAR Endometrium Interferon-gamma Jurkat Cells Decidua Humans Drug Interactions RNA Messenger fas Receptor reproductive and urinary physiology Cells Cultured Progesterone Caspase-9 Cell Death Estradiol urogenital system Tumor Necrosis Factor-alpha Cell Biology Caspase 9 Up-Regulation Enzyme Activation Antigens CD95 Hela Cells embryonic structures biology.protein Cancer research Caspase 10 Female Stromal Cells HeLa Cells |
| Zdroj: | Fluhr, H, Krenzer, S, Stein, G M, Stork, B, Deperschmidt, M, Wallwiener, D, Wesselborg, S, Zygmunt, M & Licht, P 2007, ' Interferon-gamma and tumor necrosis factor-alpha sensitize primarily resistant human endometrial stromal cells to Fas-mediated apoptosis ', Journal of Cell Science, vol. 120, no. Pt 23, pp. 4126-33 . https://doi.org/10.1242/jcs.009761 |
| ISSN: | 0021-9533 |
| Popis: | Udgivelsesdato: 2007-Dec-1 The subtle interaction between the implanting embryo and the maternal endometrium plays a pivotal role during the process of implantation. Human endometrial stromal cells (ESCs) express Fas and the implanting trophoblast cells secrete Fas ligand (FASLG, FasL), suggesting a possible role for Fas-mediated signaling during early implantation. Here we show that ESCs are primarily resistant to Fas-mediated apoptosis independently of their state of hormonal differentiation. Pre-treatment of ESCs with interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha sensitizes them to become apoptotic upon stimulation of Fas by an agonistic anti-Fas antibody. Incubation of ESCs with the early embryonic signal human chorionic gonadotropin (hCG, CGB) does not influence their reaction to Fas stimulation. The sensitizing effect of IFN-gamma and TNF-alpha was accompanied by a significant upregulation of Fas and FLICE-inhibitory protein (FLIP, CFLAR) expression in ESCs. Additionally, we observed an activation of caspase 3, caspase 8 and caspase 9 upon apoptotic Fas triggering. In summary, we demonstrate that IFN-gamma and TNF-alpha sensitize primarily apoptosis-resistant ESCs to Fas-mediated cell death. This might be due to an upregulation of Fas expression, and apoptosis seems to be mediated by active caspase 3, caspase 8 and caspase 9. The observed pro-apoptotic effect of IFN-gamma and TNF-alpha on ESCs could play an important role in the modulation of early implantation. |
| Databáze: | OpenAIRE |
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