Thyrotropin controls dolichol-linked sugar pools and oligosaccharyltransferase activity in thyroid cells
| Autor: | Jean-Louis Franc, A. Valette, Odile Chabaud, Sylvie Desruisseau |
|---|---|
| Přispěvatelé: | INSERM U38 |
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
endocrine system Glycosylation endocrine system diseases Swine medicine.medical_treatment [SDV]Life Sciences [q-bio] Thyroid Gland Mevalonic Acid Thyrotropin Stimulation Biochemistry Thyroglobulin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Dolichol Transferases Internal medicine Dolichols medicine Thyroid cells Animals Sugar Molecular Biology Cells Cultured 030304 developmental biology chemistry.chemical_classification 0303 health sciences Oligosaccharyltransferase Membrane Proteins Oligosaccharide chemistry Hexosyltransferases lipids (amino acids peptides and proteins) 030217 neurology & neurosurgery |
| Zdroj: | Molecular and Cellular Endocrinology Molecular and Cellular Endocrinology, Elsevier, 1996, 122 (2), pp.223-8 |
| ISSN: | 0303-7207 |
| Popis: | International audience; We previously showed that thyroglobulin (Tg) glycosylation is enhanced 1.5-fold under thyrotropin (TSH) stimulation, corresponding to an increased number of oligosaccharide chains per molecule of Tg. Now the steps involving dolichol components and oligosaccharyltransferase activity have been studied. Porcine thyroid cells were cultured on porous bottom filters with or without TSH and incubated with [14C]mevalonate. Under TSH regulation, the level of the whole of dolichol components was increased 1.25-fold without modifying their distribution. Dolichol, and free and monosaccharide-linked dolichyl-phosphate, represented respectively 40% and 45% of total dolichol components while dolichyl-pyrophosphate-oligosaccharide represented 3% only. A marked enhancement (4.2-fold) of oligosaccharyltransferase activity occurred in stimulated cells, which could correspond to the addition of the two TSH effects: stimulation of Tg synthesis (3-fold) and of Tg glycosylation (1.5-fold). The amount of lipid carriers appeared to be insufficiently increased but no component is a limiting step, suggesting that the turnover of dolichol derivatives may be increased under TSH control through their use by higher amounts of Tg. |
| Databáze: | OpenAIRE |
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