Biosynthesis of peptidoglycan in Staphylococcus aureus: incorporation of the Nepsilon-Ala-Lys moiety into the peptide subunit of nascent peptidoglycan
| Autor: | F C Neuhaus, J C Swenson |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Peptide Biosynthesis
Staphylococcus aureus Lysine Glycine Peptidoglycan Biology medicine.disease_cause Microbiology Acetylglucosamine chemistry.chemical_compound Biosynthesis medicine Translocase Nucleotide Molecular Biology chemistry.chemical_classification Cell-Free System Nucleotides Cell Membrane Phosphotransferases Penicillin G carbohydrates (lipids) chemistry Biochemistry biology.protein Peptides Research Article |
| Zdroj: | Journal of Bacteriology. 125:626-634 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.125.2.626-634.1976 |
| Popis: | UDP-MurNAc-Ala-DGlu-Lys(Nepsilon-Ala)-DAla-DAla was isolated from extracts of Staphylococcus aureus Copenhagen. This nucleotide accumulated in media deficient in glycine. To establish its role in peptidoglycan biosynthesis, the nucleotide-hexapeptide was compared with UDP-MurNAc-Ala-DGlu-Lys-DAla-DAla in the reaction catalyzed by phospho-MurNAc-pentapeptide translocase and in the membrane-catalyzed nascent peptidoglycan-synthetizing system. In the exchange reaction catalyzed by the translocase, the Rmax and Rmax/Km are 1.79 muM/min and 4.47 X 10(-2)/min, respectively, for UDP-MurNAc-pentapeptide and 1.81 muM/min and 4.46 X 10(-2)/min, respectively, for UDP-Mur-NAc-hexapeptide. In the synthesis of nascent peptidoglycan, the Vmax is 1.8 muM/min X 10(-2) for both the nucleotide-hexapeptide and -pentapeptide. The Vmax/Km is 5.6 X 10(-4) and 4.3 X 10(-4)/min for the nucleotide-pentapeptide and -hexapeptide, respectively. Schleifer, Hammes, and Kandler (Adv. Microb. Physiol. in press) observed that growth of S. aureus Copenhagen on a glycine-poor medium results in a peptidoglycan structure in which 20% of the lysine residues are substituted at the epsilon-amino group by L-alanine residues that do not participate in interpeptide bridge information. The in vitro studies demonstrate that UDP-MurNAc-Ala-DGlu-Lys(Nepsilon-Ala)-DAla-DAla is a possible precursor of the Nepsilon-Ala-Lys moiety. |
| Databáze: | OpenAIRE |
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