Propofol: neuroprotection in an in vitro model of traumatic brain injury
| Autor: | Joachim Weis, Mark Coburn, Rolf Rossaint, Jan Rossaint, Michael Fries, Steffen Rex |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Traumatic brain injury Hippocampal formation Pharmacology In Vitro Techniques Critical Care and Intensive Care Medicine Neuroprotection chemistry.chemical_compound Mice medicine Animals Hypnotics and Sedatives Propidium iodide Propofol Dose-Response Relationship Drug business.industry Research Hypothermia medicine.disease In vitro Dose–response relationship Disease Models Animal Neuroprotective Agents chemistry Brain Injuries medicine.symptom business medicine.drug |
| Zdroj: | Critical Care Critical care 13(R61), (2009). doi:10.1186/cc7795 |
| ISSN: | 1466-609X 1364-8535 |
| Popis: | Introduction The anaesthetic agent propofol (2,6-diisopropylphenol) has been shown to be an effective neuroprotective agent in different in vitro models of brain injury induced by oxygen and glucose deprivation. We examined its neuroprotective properties in an in vitro model of traumatic brain injury. Methods In this controlled laboratory study organotypic hippocampal brain-slice cultures were gained from six- to eight-day-old mice pups. After 14 days in culture, hippocampal brain slices were subjected to a focal mechanical trauma and subsequently treated with different molar concentrations of propofol under both normo- and hypothermic conditions. After 72 hours of incubation, tissue injury assessment was performed using propidium iodide (PI), a staining agent that becomes fluorescent only when it enters damaged cells via perforated cell membranes. Inside the cell, PI forms a fluorescent complex with nuclear DNA. Results A dose-dependent reduction of both total and secondary tissue injury could be observed in the presence of propofol under both normo- and hypothermic conditions. This effect was further amplified when the slices were incubated at 32°C after trauma. Conclusions When used in combination, the dose-dependent neuroprotective effect of propofol is additive to the neuroprotective effect of hypothermia in an in vitro model of traumatic brain injury. |
| Databáze: | OpenAIRE |
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