Microbiologic identification of bleb-related delayed-onset endophthalmitis caused by moraxella species
| Autor: | Cornut, Pierre-Loïc, Chiquet, Christophe, Bron, Alain, Romanet, Jean-Paul, Lina, Gérard, Lafontaine, Pierre-Olivier, Benito, Yvonne, Pechinot, André, Burillon, Carole, Vandenesch, François, Denis, Philippe, Renseigné, Non |
|---|---|
| Přispěvatelé: | Department of Ophtalmology, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), CHU Grenoble, Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Immunité infection vaccination (I2V), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Bactériologie, Hospices Civils de Lyon (HCL), Laboratoire de Microbiologie Médicale et Moléculaire, Université de Bourgogne (UB), Biomatériaux et remodelages matriciels, Université de Lyon-Université de Lyon, Service de physiologie digestive, urinaire, respiratoire et de l'exercice [CHU Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Hospices Civils de Lyon ( HCL ) -Hospices Civils de Lyon ( HCL ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Immunité infection vaccination ( I2V ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Hospices Civils de Lyon ( HCL ), Université de Bourgogne ( UB ), Service de physiologie digestive, urinaire, respiratoire et de l'exercice [Rouen], CHU Rouen-Hôpital Charles Nicolle [Rouen]-Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
Visual acuity genetic structures medicine.medical_treatment Antibiotics MESH : Aged MESH : Prospective Studies MESH : Vitrectomy Glaucoma Vitrectomy [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Polymerase Chain Reaction Eye Infections Bacterial Surgical Flaps Postoperative Complications 0302 clinical medicine Endophthalmitis MESH : Filtering Surgery MESH: Moraxella MESH: Aged 80 and over MESH: Postoperative Complications MESH : DNA Bacterial MESH : Female Prospective Studies MESH : Anti-Bacterial Agents MESH : Moraxellaceae Infections Moraxella species MESH : Polymerase Chain Reaction Aged 80 and over MESH: Aged 0303 health sciences MESH: Cataract Extraction MESH: Middle Aged MESH: Eye Infections Bacterial MESH: Moraxellaceae Infections Middle Aged MESH : Adult Anti-Bacterial Agents 3. Good health medicine.anatomical_structure Filtering Surgery MESH : Surgical Flaps MESH : Eye Infections Bacterial Female medicine.symptom Adult DNA Bacterial Pars plana medicine.medical_specialty MESH : Cataract Extraction medicine.drug_class Moraxellaceae Infections MESH : Male MESH: Aqueous Humor MESH : Endophthalmitis Cataract Extraction Aqueous Humor 03 medical and health sciences Ophthalmology MESH: Anti-Bacterial Agents medicine Humans Moraxella MESH: Endophthalmitis MESH : Middle Aged Bleb (cell biology) MESH : Aged 80 and over MESH: Filtering Surgery Aged MESH: Humans 030306 microbiology business.industry MESH : Humans MESH: Adult MESH: Polymerase Chain Reaction MESH: Surgical Flaps medicine.disease MESH : Aqueous Humor MESH: DNA Bacterial [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH : Moraxella eye diseases MESH: Male MESH: Prospective Studies MESH: Vitrectomy 030221 ophthalmology & optometry MESH : Postoperative Complications sense organs business MESH: Female |
| Zdroj: | Journal of Glaucoma Journal of Glaucoma, Lippincott, Williams & Wilkins, 2008, 17 (7), pp.541-5. ⟨10.1097/IJG.0b013e31816299ec⟩ Journal of Glaucoma, Lippincott, Williams & Wilkins, 2008, 17 (7), pp.541-5. 〈10.1097/IJG.0b013e31816299ec〉 |
| ISSN: | 1057-0829 |
| Popis: | International audience; PURPOSE: To report the clinical presentation and outcome of delayed-onset endophthalmitis caused by Moraxella species and to evaluate the eubacterial polymerase chain reaction (PCR) in aqueous humor samples for the microbiologic diagnosis. PATIENTS AND METHODS: Seven patients with bleb-related delayed onset-endophthalmitis caused by Moraxella were included in a prospective multicenter study (FRIENDS group, 2004 to 2005). Eubacterial PCR followed by direct sequencing and conventional cultures were carried out on aqueous humor samples taken before the first intravitreal antibiotic injection and on vitreous samples taken during pars plana vitrectomy. RESULTS: All cases were postoperative (5 after filtering surgery and 2 inadvertent filtering blebs). The mean delay of onset of endophthalmitis was 41.3 months+/-30.4 (SD) (range, 2.4 to 84.8) after surgery. Initial visual acuity was limited to light perception for 3 patients, hand motions for 2 patients, count fingers for 1 patient, and 20/125 for 1 patient. Functional recovery was variable with final visual acuity ranging from no light perception to 20/25. The eubacterial PCR carried out on aqueous humor provided microbiologic identification in all cases, whereas the cultures were negative in 6 of 7 cases. The eubacterial PCR performed on vitreous samples of 3 vitrectomized patients, after 2 intravitreal injections of antibiotics, identified Moraxella in 2 patients, whereas cultures were negative in all cases. CONCLUSIONS: Delayed-onset endophthalmitis caused by Moraxella occurs predominantly after a bleb-related infection. PCR is a more sensitive technique for the microbiologic diagnosis in this context than conventional culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|