Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis
| Autor: | M. N. Paula-Silva, Derek Felipe de Campos, Vera Maria Fonseca de Almeida-Val, Waldir Heinrichs-Caldas |
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| Rok vydání: | 2018 |
| Předmět: |
Fish Proteins
Male Time Factors Physiology 030310 physiology Aquaculture Biology Biochemistry Models Biological 03 medical and health sciences Respirometry Random Allocation Oxygen Consumption Oxidative enzyme Animals Glycolysis Muscle Skeletal Molecular Biology Lactate Dehydrogenases 030304 developmental biology 0303 health sciences Hypoxia (environmental) Gene Expression Regulation Developmental Aquatic animal Cichlids Hypoxia-Inducible Factor 1 alpha Subunit Adaptation Physiological Cell Hypoxia Cell biology Metabolic pathway Liver Organ Specificity Astronotus crassipinnis Female Energy Metabolism Anaerobic exercise |
| Zdroj: | Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 227 |
| ISSN: | 1879-1107 |
| Popis: | The aquatic habitats of the Amazon basin present dramatic variation of oxygen level, and, to survive such changes, many aquatic animals developed biochemical and physiological adaptations. The advanced teleost Astronotus crassipinnis (Perciformes) is a fish tolerant to hypoxia and known to endure such naturally variable environments. Hypoxia-Inducible factor-1α (hif-1α) is among the most important and studied genes related to hypoxia-tolerance, maintaining regular cellular function and controlling anaerobic metabolism. In the present work, we studied hif-1α expression and related it to changes in metabolic pathways of Astronotus crassipinnis exposed to 1, 3 and 5 h of hypoxia, followed by 3 h of recovery. The results show that A. crassipinnis depresses aerobic metabolic under hypoxia, with a decrease in glycolysis and oxidative enzyme activities, and increases its anaerobic metabolism with an increase in LDH activity coupled with a decrease in oxygen consumption, which indicates an increase in anaerobic capacity. In addition, the animal differentially regulates hif-1α gene in each tissue studied, with a positive relationship to its metabolic profile, suggesting that hif-1α might be one of the most important induction factors that regulate hypoxia tolerance in this species. |
| Databáze: | OpenAIRE |
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