Lazaroid U-74389G for cardioplegia-related ischemia–reperfusion injury: an experimental study
| Autor: | Efstratios Apostolakis, Georgia Kostopanagiotou, Panagiotis Dedeilias, Christos Angelidis, Georgios Giannopoulos, Maria Chorti, Apostolos Papalois, Spyridon Deftereos |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
Cardiotonic Agents Myocardial ischemia Cardiac troponin Swine Biopsy Ischemia Myocardial Reperfusion Injury 030204 cardiovascular system & hematology Creatine law.invention Necrosis Random Allocation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law medicine Cardiopulmonary bypass Animals Pregnatrienes business.industry Myocardium U 74389g medicine.disease Treatment Outcome chemistry 030220 oncology & carcinogenesis Anesthesia Surgery business Reperfusion injury Biomarkers Aminosteroid medicine.drug |
| Zdroj: | Journal of Surgical Research. 207:164-173 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2016.08.075 |
| Popis: | The adverse effects of myocardial ischemia and reperfusion during cardiopulmonary bypass (CPB) have been thoroughly described. Lazaroid U-74389G, a 21 aminosteroid, has been shown to attenuate ischemia and reperfusion injury and improve recovery in a variety of experimental models.Sixteen male swine were randomly divided in two groups. All animals underwent 45 min of ischemic cardioplegic arrest, with U-74389G addition to the standard cardioplegic solution, whereas controls underwent the same procedure without U-74389G. Creatine kinase-MB isoenzyme (CK-MB) and cardiac troponin T levels were measured immediately before CPB (time point 0), during the ischemic period (time point 1) and 30 (time point 2), 60 (time point 3), and 120 (time point 4) min after reperfusion. Myocardial biopsies were obtained at time points 0 and 4.CK-MB levels (in U/L) at time points 0-4 were 205 (186-235) versus 219 (196-269; P = 0.72), 215 (167-248) versus 253 (193-339; P = 0.23), 234 (198-255) versus 338 (249-441; P = 0.02), 244 (217-272) versus 354 (269-496; P = 0.01), and 285 (230-321) versus 439 (432-530; P 0.01) in lazaroid-treated animals versus controls, respectively. Cardiac troponin T levels (in ng/L) at time points 0-4 were 58 (26-287) versus 237 (26-395; P = 0.72), 129 (61-405) versus 265 (145-525; P = 0.23), 261 (123-467) versus 474 (427-1604; P = 0.04), 417 (204-750) versus 841 (584-1818; P = 0.11), and 643 (353-1259) versus 1600 (1378-2313; P 0.01), respectively. Necrosis grades at time point 4 were 0.0 (0.0-1.0) versus 1.5 (1.0-2.0; P 0.01) in lazaroid-treated animals versus controls, respectively.The present study, in addition to reconfirming the well-described adverse effects of CPB, demonstrates the efficacy of the newer generation lazaroid U-74389G in alleviating these effects. |
| Databáze: | OpenAIRE |
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