Dihydromyricetin inhibits cancer cell migration and matrix metalloproteinases‐2 expression in human nasopharyngeal carcinoma through extracellular signal‐regulated kinase signaling pathway
Autor: | Cheng‐Chen Huang, Chun‐Wen Su, Po‐Hui Wang, Yen‐Ting Lu, Yu‐Ting Ho, Shun‐Fa Yang, Chung‐Han Hsin, Chiao‐Wen Lin |
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Rok vydání: | 2022 |
Předmět: |
Nasopharyngeal Carcinoma
Flavonols MAP Kinase Signaling System Health Toxicology and Mutagenesis Nasopharyngeal Neoplasms General Medicine Management Monitoring Policy and Law Toxicology Cell Movement Cell Line Tumor Humans Matrix Metalloproteinase 2 Neoplasm Invasiveness Extracellular Signal-Regulated MAP Kinases Signal Transduction |
Zdroj: | Environmental Toxicology. 37:1244-1253 |
ISSN: | 1522-7278 1520-4081 |
Popis: | Nasopharyngeal carcinoma (NPC) is endemic in Southeast Asia and the main cause of treatment failure is metastasis. A lot of biological and pharmacological actions of dihydromyricetin (DHM) have been reported such as regulating glucose and anti-cancer effects. The effects of DHM on the cancer invasion and migration of NPC, however, are still unclear. We therefore investigated the in vitro anti-metastatic properties of DHM on three human NPC cell lines (HONE-1, NPC-39, and NPC-BM), as well as the underlying signaling pathways. Our study revealed that DHM could suppress the migration and invasion in NPC cells. Gelatin zymography assay and western blotting assays demonstrated that DHM suppressed the enzyme activity and protein expression of matrix metalloproteinases-2 (MMP-2). Mitogen-activated protein kinases were also investigated to elucidate the signaling pathway, which showed that phosphorylation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) was inhibited after the treatment of DHM. In conclusion, our data revealed that DHM inhibited the migration and invasion of NPC cells by suppressing the expression of MMP-2 via down regulating the ERK1/2 signaling pathway. |
Databáze: | OpenAIRE |
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