Purification of paraoxonase enzyme from the sera of patients with Behcet's disease and analyzing the effects of the drugs containing imuran (azathioprine), prednisolone (methylprednisolone) and colchium (colchicine)
| Autor: | Asuman Ozkan, Ceyhun Işık, Yaşar Demir, Esen Tasgin, Nazan Demir, Hayrunnisa Nadaroglu |
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| Přispěvatelé: | Bayburt University |
| Rok vydání: | 2014 |
| Předmět: |
enzyme assay
Clinical Biochemistry Pharmaceutical Science Azathioprine Behcet's disease Pharmacology Arylesterase chemistry.chemical_compound Colchicine Pharmacology (medical) PON1 protein human Enzyme Inhibitors Purification biology pH Behcet Syndrome electrophoretic mobility Methylprednisolone priority journal Enzyme inhibitor Paraoxonase Prednisolone medicine.drug drug purification in vitro study enzymology enzyme inhibitor Article blood medicine Humans controlled study human antagonists and inhibitors business.industry arylesterase Aryldialkylphosphatase Biochemistry (medical) temperature case control study medicine.disease major clinical study Kinetics chemistry drug blood level Case-Control Studies Immunology biology.protein sepharose business Behcet disease protein determination maximum reaction velocity polyacrylamide gel electrophoresis |
| Zdroj: | Drug metabolism letters. 8(1) |
| ISSN: | 1874-0758 |
| Popis: | In this study, serum samples from 50 patients with the diagnosis of Behcet’s disease and 20 healthy volunteers were analyzed. The study consists of three parts. In the first part, paraoxonase (PON) activities were determined in the serum samples of 50 patients with Behcet’s disease and 20 healthy people. In the second part, equal volumes of serum samples from 50 patients were pooled and PON enzymes were purified by using Sepharose-4B-L-tyrosine tyrosine-1-naphtylamine affinity column. Optimum temperature, optimum pH, Vmax and Km values of the pure enzymes were determined. The same purification procedure was also performed in the serum samples of 20 healthy people. Electrophoretic mobility was observed (via SDS-PAGE) in the PON enzymes that were purified from the serum samples of patients with Behcet’s disease and healthy people. In the third part, in vitro effects of drugs containing azathioprine, methylprednisolone and colchicine that have already been used for the treatment of Behcet’s disease were tested on the PON enzymes of the patients with Behcet’s disease and control group. IC50 values and Ki constant values were measured and inhibition types were determined for the drugs containing azathioprine, methylprednisolone and colchicine that have already been used for the treatment of the Behcet’s disease and demonstrate in vitro inhibition effects. ©2014 Bentham Science Publishers. |
| Databáze: | OpenAIRE |
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