Multiple biological activities of human recombinant interleukin 1
| Autor: | G LoPreste, H A Bernheim, Philip E. Auron, R N Love, Andrew C. Webb, R C Reuben, James W. Mier, Charles A. Dinarello, D L Lynn, Joseph G. Cannon |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Interleukin 2
Fever T-Lymphocytes T cell Recombinant Interleukin Biology Lymphocyte Activation Dinoprostone law.invention Mice law Escherichia coli medicine Animals Humans Amino Acids Prostaglandin E2 Mice Inbred C3H Dose-Response Relationship Drug Prostaglandins E Monocyte Interleukin DNA General Medicine Fibroblasts Molecular biology Recombinant Proteins In vitro Molecular Weight medicine.anatomical_structure Recombinant DNA Rabbits Interleukin-1 Research Article medicine.drug |
| Zdroj: | Journal of Clinical Investigation. 77:1734-1739 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci112495 |
| Popis: | Complementary DNA coding for human monocyte interleukin 1 (IL-1), pI 7 form, was expressed in Escherichia coli. During purification, IL-1 activity on murine T cells was associated with the recombinant protein. Homogeneous human recombinant IL-1 (hrIL-1) was tested in several assays to demonstrate the immunological and inflammatory properties attributed to this molecule. hrIL-1 induced proliferative responses in a cloned murine T cell in the presence of suboptimal concentrations of mitogen, whereas no effect was observed with hrIL-1 alone. At concentrations of 0.05 ng/ml, hrIL-1 doubled the response to mitogen (5 X 10(6) half maximal units/mg). Human peripheral blood T cells depleted of adherent cells underwent a blastogenic response and released interleukin 2 in the presence of hrIL-1 and mitogen. hrIL-1 was a potent inflammatory agent by its ability to induce human dermal fibroblast prostaglandin E2 production in vitro and to produce monophasic (endogenous pyrogen) fever when injected into rabbits or endotoxin-resistant mice. These studies establish that the dominant pI 7 form of recombinant human IL-1 possesses immunological and inflammatory properties and acts on the central nervous system to produce fever. |
| Databáze: | OpenAIRE |
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