Genetic diversity of root nodule bacteria nodulating Lotus corniculatus and Anthyllis vulneraria in Sweden
Autor: | Osei Yaw Ampomah, Kerstin Huss-Danell |
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Rok vydání: | 2011 |
Předmět: |
DNA
Bacterial Molecular Sequence Data DNA Ribosomal Applied Microbiology and Biotechnology Microbiology Anthyllis vulneraria Rhizobia Bacterial Proteins 23S ribosomal RNA RNA Ribosomal 16S DNA Ribosomal Spacer Botany Cluster Analysis Lotus corniculatus Internal transcribed spacer Phylogeny Ecology Evolution Behavior and Systematics Sweden Genetic diversity Bacteria biology Genetic Variation food and beverages Fabaceae Biodiversity Sequence Analysis DNA biology.organism_classification Bacterial Typing Techniques Mesorhizobium loti Molecular Typing Restriction fragment length polymorphism Root Nodules Plant Acyltransferases Polymorphism Restriction Fragment Length |
Zdroj: | Systematic and Applied Microbiology. 34:267-275 |
ISSN: | 0723-2020 |
Popis: | Very little is known about the genetic diversity and phylogeny of rhizobia nodulating Lotus species in northern temperate regions. We have therefore studied the genetic diversity among a total of 61 root nodule bacteria isolated from Lotus corniculatus and Anthyllis vulneraria from different geographic sites and habitats in Sweden by restriction fragment length polymorphism (RFLP) of the internal transcribed spacer between their 16S rRNA and 23S rRNA (IGS) region. A high diversity consisting of 26 IGS types from 54 L. corniculatus isolates and five IGS types from seven A. vulneraria isolates was found. The 16S rRNA sequences and phylogeny of representatives of the different IGS types showed four interesting exceptions from the majority of the isolates belonging to the genus Mesorhizobium: Two isolates were both found to be closely related to Rhodococcus spp., and two other isolates showed close relationship with Geobacillus spp. and Paenibacillus spp., respectively. The nodA sequences and phylogeny showed that all the isolates, including those not belonging to the traditional rhizobia genera, harbored nodA sequences which were typical of Mesorhizobium loti. Generally, the 16S rRNA and nodA phylogenetic trees were not congruent in that isolates with similar 16S rRNA sequences were associated with isolates harboring different nodA sequences. All the isolates were confirmed to nodulate L. corniculatus in an inoculation test. This is the first report of members of these non-rhizobia genera being able to nodulate legumes, and we suggest that they may have acquired their nodulating properties through lateral gene transfer. |
Databáze: | OpenAIRE |
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