Structural characterization and antioxidant activities of polysaccharides from Citrus aurantium L
| Autor: | Zun Peng Shu, Wen Juan Jiao, Na Xing, Qiu-Hong Wang, Hai-Xue Kuang, Bing-You Yang, Bing Qing Xu |
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| Rok vydání: | 2014 |
| Předmět: |
Citrus
Antioxidant medicine.medical_treatment Composition analysis Polysaccharide Biochemistry Antioxidants Polysaccharides Structural Biology In vivo medicine Humans Organic chemistry Food science Molecular Biology chemistry.chemical_classification Ion exchange Plant Extracts Chemistry Atomic force microscopy Extraction (chemistry) Free Radical Scavengers General Medicine In vitro Chromatography Gel Oxidation-Reduction |
| Zdroj: | International Journal of Biological Macromolecules. 67:112-123 |
| ISSN: | 0141-8130 |
| Popis: | Three polysaccharide fractions were obtained from Citrus aurantium L. (CAL) by sequential extraction with cold water, hot water, and 1.0M NaOH, respectively. The fractions were denoted CALA, CALB, and CALC. Structural characterization was conducted by physicochemical property, FTIR, and SEM analyses. Antioxidant activities in vivo and in vitro were also evaluated. CALB, which showed the highest activity, was further isolated to afford four purified polysaccharides (CALB-1-4) by various ion exchange and gel-filtration chromatography. Meanwhile, the purified polysaccharides were subjected to composition analysis and screened by antioxidant activity in vitro. Among the four purified polysaccharides, CALB-3 had the highest antioxidant activity and its structure was analyzed by FTIR, SEM and AFM microscopy. Overall, these results indicated that polysaccharides from CAL had potential therapeutic applications in the medical and food industries because of their antioxidant activities. |
| Databáze: | OpenAIRE |
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