Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation
| Autor: | Raquel Osorio, Ruoqiong Chen, Fátima S. Aguilera, Conrado Aparicio, Estrella Osorio, Antonio L. Medina-Castillo, Sergio Acosta, Manuel Toledano-Osorio, Manuel Toledano |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
inorganic chemicals
Polymers education 0206 medical engineering Biomedical Engineering chemistry.chemical_element Nanoparticle 02 engineering and technology Zinc dentin Biochemistry Mineralization (biology) biofilm Biomaterials chemistry.chemical_compound stomatognathic system Tensile Strength Materials Testing Dentin medicine Viability assay Molecular Biology health care economics and organizations Remineralisation nanoparticle technology industry and agriculture Biofilm Dental Bonding General Medicine 021001 nanoscience & nanotechnology Phosphate 020601 biomedical engineering Resin Cements medicine.anatomical_structure chemistry Biofilms Dentin-Bonding Agents Nanoparticles interface 0210 nano-technology degradation Biotechnology Nuclear chemistry |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname |
| Popis: | The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups of polymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were applied after dentin etching. The disks were exposed to a cariogenic biofilm challenge in a Drip-Flow Reactor during 72 h and 7 d. Half of the specimens were not subjected to biofilm formation but stored 72 h and 7 d. LIVE/DEAD® viability assay, nano-dynamic mechanical assessment, Raman spectroscopy and field emission electron microscopy (FESEM) analysis were performed. The measured bacterial death rates, at 7 d were 46% for the control group, 51% for the undoped-NPs, 32% for Dox-NPs, and 87% for Zn-NPs; being total detected bacteria reduced five times in the Dox-NPs group. Zn-NPs treated samples reached, in general, the highest complex modulus values at the resin-dentin interface over time. Regarding the mineral content, Zn-NPs-treated dentin interfaces showed the highest mineralization degree associated to the phosphate peak and the relative mineral concentration. FESEM images after Zn-NPs application permitted to observe remineralization of the etched and non-resin infiltrated collagen layer, and bacteria were scarcely encountered. The combined antibacterial and remineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Dox-NPs exerted an antibacterial role but did not remineralize the bonded interface. Undoped-NPs did not improve the properties of the interfaces. Application of Zn-doped NPs during the bonding procedure is encouraged. STATEMENT OF SIGNIFICANCE: Application of Zn-doped nanoparticles on acid etched dentin reduced biofilm formation and viability at the resin-dentin interface due to both remineralization and antibacterial properties. Doxycycline-doped nanoparticles also diminished oral biofilm viability, but did not remineralize the resin-dentin interface. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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