Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues
| Autor: | Rovilson Gilioli, Carol Benatti, Mariana Vitelo Xavier, André Luiz Jardini, Rubens Maciel Filho, Nádson M.N. Lima, Flavio Manenti, Lamia Zuñiga Liñan, Ana Amélia Rodrigues |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
synthesis lcsh:Biotechnology Mesenchymal cells 02 engineering and technology Bone tissue 01 natural sciences chemistry.chemical_compound 010608 biotechnology lcsh:TP248.13-248.65 medicine Methyl methacrylate Cytotoxicity chemistry.chemical_classification Multidisciplinary viability mesenchymal cells technology industry and agriculture Solution polymerization Polymer 021001 nanoscience & nanotechnology Poly(methyl methacrylate) PMMA Calcein Monomer medicine.anatomical_structure chemistry visual_art visual_art.visual_art_medium cytotoxicity 0210 nano-technology Nuclear chemistry |
| Zdroj: | Brazilian Archives of Biology and Technology, Volume: 61, Article number: e18160526, Published: 28 JUN 2018 Brazilian Archives of Biology and Technology, Vol 61, Iss 0 (2018) Brazilian Archives of Biology and Technology v.61 2018 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
| Popis: | A cytotoxicity study is performed on a poly(methyl methacrylate) polymer (PMMA) to be used for the fabrication of bone tissue by Rapid Prototyping (RP). The solution polymerization is conducted in a pilot plant reactor using more appropriated reagents in consideration of the medical application. Moreover, the polymer is efficiently handled to avoid the side effect of the monomer, reducing the concentration of this specie to 287,731 µg MMA/kg PMMA. The cytotoxicity of the polymer is determined through growth monitoring, adherence and morphology of L-929 cells. Additionally, MTT and LIVE/DEAD tests are performed. The results showed continuous and progressive growth of the cells on the surface of the specimens. Moreover, the material did not influence on the viability of mesenchymal cells and inverted fluorescence microscopy images showed a polyanionic dye calcein well retained in the cells in contact with the PMMA as well as the negative control after 72 hours. Thus, the polymer was efficiently synthesized and handled for the expected demands. |
| Databáze: | OpenAIRE |
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