Design and Construction of Large Amyloid Fibers
| Autor: | Justin R. Barone, Devin M. Ridgley, Caitlin M. W. Rippner |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adder
Materials science Morphology (linguistics) Beta sheet Modulus 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials lcsh:TP890-933 lcsh:TP200-248 Fiber Composite material lcsh:QH301-705.5 Civil and Structural Engineering Alanine chemistry.chemical_classification technology industry and agriculture amyloid lcsh:Chemicals: Manufacture use etc musculoskeletal system 021001 nanoscience & nanotechnology lcsh:QC1-999 0104 chemical sciences 3. Good health Amino acid lcsh:Biology (General) β-sheet chemistry Mechanics of Materials Ceramics and Composites lcsh:Textile bleaching dyeing printing etc protein 0210 nano-technology Amyloid (mycology) lcsh:Physics fiber |
| Zdroj: | Fibers Volume 3 Issue 2 Pages 90-102 Fibers, Vol 3, Iss 2, Pp 90-102 (2015) |
| ISSN: | 2079-6439 |
| DOI: | 10.3390/fib3020090 |
| Popis: | Mixtures of “template” and “adder” proteins self-assemble into large amyloid fibers of varying morphology and modulus. Fibers range from low modulus, rectangular cross-sectioned tapes to high modulus, circular cross-sectioned cylinders. Varying the proteins in the mixture can elicit “in-between” morphologies, such as elliptical cross-sectioned fibers and twisted tapes, both of which have moduli in-between rectangular tapes and cylindrical fibers. Experiments on mixtures of proteins of known amino acid sequence show that control of the large amyloid fiber morphology is dependent on the amount of glutamine repeats or “Q-blocks” relative to hydrophobic side chained amino acids such as alanine, isoleucine, leucine, and valine in the adder protein. Adder proteins with only hydrophobic groups form low modulus rectangular cross-sections and increasing the Q-block content allows excess hydrogen bonding on amide groups that results in twist and higher modulus. The experimental results show that large amyloid fibers of specific shape and modulus can be designed and controlled at the molecular level. |
| Databáze: | OpenAIRE |
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