miR-146a-5p and miR-193a-5p Synergistically Inhibited the Proliferation of Human Colorectal Cancer Cells (HT-29 cell line) through ERK Signaling Pathway
| Autor: | Behzad Baradaran, Dariush Shanehbandi, Behzad Mansoori, Ahad Mokhtarzadeh, Khalil Hajiasgharzadeh, Vahid Khaze Shahgoli, Amir Baghbanzadeh Kojabad, Saeed Noorolyai, Elham Baghbani |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
erk pathway Pharmaceutical Science colorectal cancer 02 engineering and technology RM1-950 Biology mirna-146a-5p 030226 pharmacology & pharmacy Metastasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine microRNA medicine cell growth DAPI General Pharmacology Toxicology and Pharmaceutics Cell growth Cell migration 021001 nanoscience & nanotechnology medicine.disease chemistry Apoptosis Cancer cell Cancer research mirna-193a-5p Therapeutics. Pharmacology 0210 nano-technology Research Article |
| Zdroj: | Advanced Pharmaceutical Bulletin, Vol 11, Iss 4, Pp 755-764 (2021) Advanced Pharmaceutical Bulletin |
| ISSN: | 2251-7308 2228-5881 |
| Popis: | Purpose: The expression of miR-146a-5p and miR-193a-5p in colorectal cancer (CRC) is associated with cancer development, metastasis, and reduced survival rate of the tumor-suffered subjects. This examination aimed to assess the impact of these microRNAs (miRNAs) in CRC and their mechanisms in the proliferation and migration of cancer cells. Methods: miR-146a-5p and -193a-5p were transfected into the HT-29 cell line and assessed their impact on metastasis-related genes. The synergistic effects of these miRNAs on migration were evaluated by wound healing approach. To assess the influence of these miRNAs on the proliferation of and apoptosis of cells, the MTT test, annexin V staining test, and DAPI staining test were done. Then, the protein expression of extracellular-signal-regulated kinase (ERK) and phosphorylated ERK (p-ERK) were investigated. Results: miR-146a-5p and-193a-5p could inhibit the CRC cells proliferation, and could synergistically induce apoptosis in CRC cells, and also repressed cell migration, and could reduce p-ERK expression. Conclusion: miR-146a-5p and-193a-5p have an important role in cell viability and proliferation via ERK signaling pathway. Thus, the simultaneous use of these miRNAs may be suggested as a probable therapeutic strategy in this cancer therapy. |
| Databáze: | OpenAIRE |
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